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Product Info Summary
| SKU: | LS001739 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of discs, large homolog 1 (Drosophila) (DLG1), transcript variant 2 |
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Product info
Product Name
SAP97 (DLG1) (NM_004087) Human Over-expression Lysate
SKU/Catalog Number
LS001739
Size
100 µg
Description
Transient overexpression lysate of discs, large homolog 1 (Drosophila) (DLG1), transcript variant 2
Storage & Handling
Cite This Product
SAP97 (DLG1) (NM_004087) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS001739)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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Protein Target Info & Infographic
Gene/Protein Information for DLG1 (Source: Uniprot.org, NCBI)
Gene Name
DLG1
Full Name
Disks large homolog 1
Weight
100.455kDa
Superfamily
MAGUK family
Alternative Names
discs, large homolog 1 (Drosophila); disks large homolog 1; DKFZp761P0818; DKFZp781B0426; hDlg; large (Drosophila) homolog 1; presynaptic protein SAP97 DLG1 DLGH1, SAP-97, SAP97, hdlg discs large MAGUK scaffold protein 1 disks large homolog 1|dJ1061C18.1.1|discs large homolog 1, scribble cell polarity complex component|presynaptic protein SAP97|synapse-associated protein 97
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on DLG1, check out the DLG1 Infographic
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In this infographic, you will see the following information for DLG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For SAP97 (DLG1) (NM_004087) Human Over-expression Lysate (LS001739)
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