SEMCAP3 (PDZRN3) (NM_015009) Human Over-expression Lysate

Product Name

SEMCAP3 (PDZRN3) (NM_015009) Human Over-expression Lysate

See all PDZRN3 products

SKU/Catalog Number

LS023024

Size

100 µg

Description

Transient overexpression lysate of PDZ domain containing ring finger 3 (PDZRN3)

Storage & Handling

Cite This Product

SEMCAP3 (PDZRN3) (NM_015009) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS023024)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

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Gene/Protein Information for PDZRN3 (Source: Uniprot.org, NCBI)

Alternative Names

E3 ubiquitin-protein ligase PDZRN3; EC 6.3.2.-; Ligand of Numb protein X 3; likely ortholog of mouse semaF cytoplasmic domain associated protein 3; LNX3KIAA1095SEMACAP3; PDZ domain containing ring finger 3; PDZ domain-containing RING finger protein 3; Protein SEMACAP3; Semaphorin cytoplasmic domain-associated protein 3; SEMCAP3 PDZRN3 LNX3, SEMACAP3, SEMCAP3 PDZ domain containing ring finger 3 E3 ubiquitin-protein ligase PDZRN3|RING-type E3 ubiquitin transferase PDZRN3|ligand of Numb protein X 3|likely ortholog of mouse semaF cytoplasmic domain associated protein 3|semaphorin cytoplasmic domain-associated protein 3

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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