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Product Info Summary
| SKU: | LS006456 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of SH3-domain GRB2-like 2 (SH3GL2) |
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Product info
Product Name
SH3GL2 (NM_003026) Human Over-expression Lysate
See all Endophilin A1/SH3GL2 products
SKU/Catalog Number
LS006456
Size
100 µg
Description
Transient overexpression lysate of SH3-domain GRB2-like 2 (SH3GL2)
Storage & Handling
Cite This Product
SH3GL2 (NM_003026) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006456)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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SH3GL2 Human Over-expression Lysates NM_003026.
Western blot validation of overexpression lysate (LS006456) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SH3GL2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for SH3GL2 (Source: Uniprot.org, NCBI)
Gene Name
SH3GL2
Full Name
Endophilin-A1
Weight
39.962kDa
Superfamily
endophilin family
Alternative Names
CNSA2; CNSA2FLJ25015; EEN-B1; EEN-B1bA335L15.1 (SH3-domain GRB2-like 2); Endophilin A1; Endophilin-1; endophilin-A1; FLJ20276; SH3 domain protein 2A; SH3 domain-containing GRB2-like protein 2; SH3D2A; SH3D2AEndophilin A1 BAR domain; SH3-domain GRB2-like 2; SH3GL2; SH3P4; SH3P4endophilin-1 SH3GL2 CNSA2, EEN-B1, SH3D2A, SH3P4 SH3 domain containing GRB2 like 2, endophilin A1 endophilin-A1|Endophilin A1 BAR domain|SH3 domain protein 2A|SH3 domain-containing GRB2-like protein 2|SH3-domain GRB2-like 2|bA335L15.1 (SH3-domain GRB2-like 2)|endophilin-1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on SH3GL2, check out the SH3GL2 Infographic
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In this infographic, you will see the following information for SH3GL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For SH3GL2 (NM_003026) Human Over-expression Lysate (LS006456)
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