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Product Info Summary
| SKU: | LS006573 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of solute carrier family 19 (folate transporter), member 1 (SLC19A1) |
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Product info
Product Name
SLC19A1 (NM_194255) Human Over-expression Lysate
See all Reduced Folate Carrier/SLC19A1 products
SKU/Catalog Number
LS006573
Size
100 µg
Description
Transient overexpression lysate of solute carrier family 19 (folate transporter), member 1 (SLC19A1)
Storage & Handling
Cite This Product
SLC19A1 (NM_194255) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006573)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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SLC19A1 Human Over-expression Lysates NM_194255.
Western blot validation of overexpression lysate (LS006573) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SLC19A1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for SLC19A1 (Source: Uniprot.org, NCBI)
Gene Name
SLC19A1
Full Name
Reduced folate transporter
Weight
64.868kDa
Superfamily
reduced folate carrier (RFC) transporter (TC 2.A.48) family
Alternative Names
CHMD; FLOT1; folate transporter 1; FOLT; FOLThuman reduced folate carrier (RFC)10Intestinal folate carrier 1; IFC1; IFC-1; Placental folate transporter; Reduced folate carrier protein; REFC; RFC; RFC1; SLC19A1; solute carrier family 19 (folate transporter), member 1; Solute carrier family 19 member 1 SLC19A1 CHMD, FOLT, IFC-1, IFC1, REFC, RFC, RFC1, RFT-1, hRFC, hSLC19A1 solute carrier family 19 member 1 reduced folate transporter|folate transporter 1|intestinal folate carrier 1|placental folate transporter|reduced folate carrier 1|reduced folate carrier protein|reduced folate transporter 1|solute carrier family 19 (folate transporter), member 1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on SLC19A1, check out the SLC19A1 Infographic
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In this infographic, you will see the following information for SLC19A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For SLC19A1 (NM_194255) Human Over-expression Lysate (LS006573)
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No publications found for LS006573
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