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Product Info Summary
| SKU: | PB9504 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SLC19A1 Antibody Picoband®
SKU/Catalog Number
PB9504
PB0534 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SLC19A1 Antibody Picoband® catalog # PB9504. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SLC19A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9504)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human SLC19A1.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9504 is reactive to SLC19A1 in Human, Monkey, Mouse, Rat
Observed Molecular Weight
65 kDa
Calculated molecular weight
64.9 kDa
Background of SLC19A1
Solute carrier family 19 (folate transporter), member 1, also known as SLC19A1 or RFC1, is a protein which in humans is encoded by the SLC19A1 gene. Transport of folate compounds into mammalian cells can occur via receptor-mediated or carrier-mediated mechanisms. A functional coordination between these 2 mechanisms has been proposed to be the method of folate uptake in certain cell types. Methotrexate (MTX) is an antifolate chemotherapeutic agent that is actively transported by the carrier-mediated uptake system. And RFC1 plays a role in maintaining intracellular concentrations of folate.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9504 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat, Monkey
Positive Control
WB: rat brain tissue, rat ovary tissue, mouse brain tissue, mouse testicular tissue, mouse ovary tissue, mouse NIH/3T3 whole cell, human placenta tissue, human HEK293 whole cell, monkey COS-7 whole cell, human PC-3 whole cell, human 22RV1 whole cell, human K562 whole cell
Validation Images & Assay Conditions
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Western blot analysis of SLC19A1 using anti-SLC19A1 antibody (PB9504).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat ovary tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse testicular tissue lysates,
Lane 5: mouse ovary tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates,
Lane 7: human placenta tissue lysates,
Lane 8: human HEK293 whole cell lysates,
Lane 9: monkey COS-7 whole cell lysates,
Lane 10: human PC-3 whole cell lysates,
Lane 11: human 22RV1 whole cell lysates,
Lane 12: human K562 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC19A1 antigen affinity purified polyclonal antibody (Catalog # PB9504) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC19A1 at approximately 65KD. The expected band size for SLC19A1 is at 65KD.
Specific Publications For Anti-SLC19A1 Antibody Picoband® (PB9504)
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1 Customer Q&As for Anti-SLC19A1 Antibody Picoband®
Question
We are currently using anti-SLC19A1 antibody PB9504 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
D. Jha
Verified customer
Asked: 2014-10-08
Answer
The anti-SLC19A1 antibody (PB9504) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-10-08
