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Product Info Summary
| SKU: | EKC2510 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human, Mouse |
| Application: | ELISA |
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Product info
Product Name
SMC1 (Phospho-Ser957) Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC2510
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The SMC1 (Phospho-Ser957) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor SMC1 (Phospho-Ser957) protein expression profile in cells. The kit can be used for measuring the relative amounts of SMC1 (Phospho-Ser957) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on SMC1 (Phospho-Ser957).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
SMC1 (Phospho-Ser957) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2510)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2510 is reactive to SMC1A in Human, Mouse samples
Application Guarantee
EKC2510 is guaranteed for ELISA in Human, Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-SMC1A (Phospho-specific) Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-SMC1A Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
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Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from HuvEc cells treated with EGF 200ng/ml 5'/HeLa cells treated with EGF 200ng/ml 15'
Protein Target Info & Infographic
Gene/Protein Information For SMC1A (Source: Uniprot.Org, NCBI)
Gene Name
SMC1A
Full Name
Structural maintenance of chromosomes protein 1A
Weight
143.233kDa
Superfamily
SMC family
Alternative Names
CDLS2; DXS423ESMC1 structural maintenance of chromosomes 1-like 1 (yeast); KIAA0178DKFZp686L19178; SB1.8; SB1.8MGC138332; segregation of mitotic chromosomes 1; SMC protein 1A; SMC1 (structural maintenance of chromosomes 1, yeast)-like 1; SMC1 structural maintenance of chromosomes 1-like 1; SMC1; SMC1A; SMC-1A; SMC-1-alpha; SMC1L1; SMC1SMC1alpha; Smcb; structural maintenance of chromosomes 1A; structural maintenance of chromosomes protein 1A SMC1A CDLS2, DEE85, DXS423E, EIEE85, SB1.8, SMC1, SMC1L1, SMC1alpha, SMCB structural maintenance of chromosomes 1A structural maintenance of chromosomes protein 1A|SMC protein 1A|SMC-1-alpha|SMC1 (structural maintenance of chromosomes 1, yeast)-like 1|epididymis secretory sperm binding protein|segregation of mitotic chromosomes 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SMC1A, check out the SMC1A Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SMC1A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For SMC1 (Phospho-Ser957) Colorimetric Cell-Based ELISA Kit (EKC2510)
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