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Product Info Summary
| SKU: | M02148 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SMC1 SMC1A Rabbit Monoclonal Antibody
SKU/Catalog Number
M02148
BM4655 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SMC1 SMC1A Rabbit Monoclonal Antibody catalog # M02148. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SMC1 SMC1A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02148)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GHE-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SMC1
Reactive Species
M02148 is reactive to SMC1A in Human, Mouse, Rat
Observed Molecular Weight
143 kDa
Calculated molecular weight
143.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02148 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: HeLa cell, Jurkat cell
Validation Images & Assay Conditions
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Western blot analysis of SMC1 expression in (1) HeLa cell lysate; (2) Jurkat cell lysate.
Specific Publications For Anti-SMC1 SMC1A Rabbit Monoclonal Antibody (M02148)
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Customer Q&As
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7 Customer Q&As for Anti-SMC1 SMC1A Rabbit Monoclonal Antibody
Question
Does M02148 anti-SMC1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
It shows on the product datasheet, M02148 anti-SMC1 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-23
Question
Will anti-SMC1 Rabbit Monoclonal antibody M02148 work for WB with embryonic kidney?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
According to the expression profile of embryonic kidney, SMC1A is highly expressed in embryonic kidney. So, it is likely that anti-SMC1 Rabbit Monoclonal antibody M02148 will work for WB with embryonic kidney.
Boster Scientific Support
Answered: 2019-11-13
Question
Is this M02148 anti-SMC1 Rabbit Monoclonal antibody reactive to the isotypes of SMC1A?
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
The immunogen of M02148 anti-SMC1 Rabbit Monoclonal antibody is A synthesized peptide derived from human SMC1 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-17
Question
Please see the WB image, lot number and protocol we used for embryonic kidney using anti-SMC1 Rabbit Monoclonal antibody M02148. Please let me know if you require anything else.
G. Jha
Verified customer
Asked: 2019-04-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-09
Question
Is a blocking peptide available for product anti-SMC1 Rabbit Monoclonal antibody (M02148)?
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
We do provide the blocking peptide for product anti-SMC1 Rabbit Monoclonal antibody (M02148). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-07-11
Question
I was wanting to use to test anti-SMC1 Rabbit Monoclonal antibody M02148 on mouse embryonic kidney for research purposes, then I may be interested in using anti-SMC1 Rabbit Monoclonal antibody M02148 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Miller
Verified customer
Asked: 2014-08-20
Answer
The products we sell, including anti-SMC1 Rabbit Monoclonal antibody M02148, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-08-20
Question
Does anti-SMC1 Rabbit Monoclonal antibody M02148 work on canine WB with leukemic t-cell?
K. Thomas
Verified customer
Asked: 2014-03-14
Answer
Our lab technicians have not validated anti-SMC1 Rabbit Monoclonal antibody M02148 on canine. You can run a BLAST between canine and the immunogen sequence of anti-SMC1 Rabbit Monoclonal antibody M02148 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine leukemic t-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-03-14
