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Product Info Summary
| SKU: | MP02148 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-SMC1 (S957) Monoclonal Antibody
SKU/Catalog Number
MP02148
BM4505 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-SMC1 (S957) Monoclonal Antibody catalog # MP02148. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-SMC1 (S957) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MP02148)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FCI-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-SMC1 (S957) Structural maintenance of chromosomes 1 (SMC1) protein is a chromosomal protein member of the cohesin complex that enables sister chromatid cohesion and plays a role in DNA repair . ATM/NBS1-dependent phosphorylation of SMC1 occurs at Ser957 and Ser966 in response to ionizing radiation (IR) as part of the intra-S-phase DNA damage checkpoint. SMC1 phosphorylation is ATM-independent in cells subjected to other forms of DNA damage, including UV light and hydroxyurea treatment.
Reactive Species
MP02148 is reactive to SMC1A in Human
Observed Molecular Weight
160 kDa
Calculated molecular weight
143.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MP02148 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: HeLa cell
IHC: human bladder cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of Phospho-SMC1 (S957) expression in (1) HeLa cell lysate; (2) Hela cells lysate treated with AP.
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Immunohistochemical analysis of paraffin-embedded human bladder cancer, using Phospho-SMC1 (S957) Antibody.
Specific Publications For Anti-Phospho-SMC1 (S957) Monoclonal Antibody (MP02148)
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