Product Info Summary
SKU: | LS007108 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of transmembrane 7 superfamily member 2 (TM7SF2) |
Product info
Product Name
TM7SF2 (NM_003273) Human Over-expression Lysate
SKU/Catalog Number
LS007108
Size
100 µg
Description
Transient overexpression lysate of transmembrane 7 superfamily member 2 (TM7SF2)
Storage & Handling
Cite This Product
TM7SF2 (NM_003273) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS007108)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
Click image to see more details
TM7SF2 Human Over-expression Lysates NM_003273.
Western blot validation of overexpression lysate (LS007108) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with TM7SF2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for TM7SF2 (Source: Uniprot.org, NCBI)
Gene Name
TM7SF2
Full Name
Delta(14)-sterol reductase TM7SF2
Weight
46.406kDa
Superfamily
ERG4/ERG24 family
Alternative Names
ANG1; C-14 sterol reductase; delta(14)-sterol reductase; delta-14-SR; DHCR14A; NET47; putative sterol reductase SR-1; sterol C14-reductase; transmembrane 7 superfamily member 2 TM7SF2 ANG1, C14SR, DHCR14A, NET47 transmembrane 7 superfamily member 2 delta(14)-sterol reductase TM7SF2|3-beta-hydroxysterol Delta (14)-reductase|C-14 sterol reductase|another new gene 1 protein|delta-14-SR|putative sterol reductase SR-1|sterol C14-reductase
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on TM7SF2, check out the TM7SF2 Infographic
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In this infographic, you will see the following information for TM7SF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For TM7SF2 (NM_003273) Human Over-expression Lysate (LS007108)
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No publications found for LS007108
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