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Product Info Summary
| SKU: | LS023671 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of transmembrane protein with EGF-like and two follistatin-like domains 2 (TMEFF2) |
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Product info
Product Name
TMEFF2 (NM_016192) Human Over-expression Lysate
See all TMEFF2/Tomoregulin-2 products
SKU/Catalog Number
LS023671
Size
100 µg
Description
Transient overexpression lysate of transmembrane protein with EGF-like and two follistatin-like domains 2 (TMEFF2)
Storage & Handling
Cite This Product
TMEFF2 (NM_016192) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS023671)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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TMEFF2 Human Over-expression Lysates NM_016192.
Western blot validation of overexpression lysate (LS023671) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with TMEFF2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for TMEFF2 (Source: Uniprot.org, NCBI)
Gene Name
TMEFF2
Full Name
Tomoregulin-2
Weight
41.428kDa
Superfamily
tomoregulin family
Alternative Names
CT120.2; HPP1member 2; Hyperplastic polyposis protein 1; TENB2; TMEFF2; Tomoregulin2; Tomoregulin-2; TPEFtomoregulin; TR-2; transmembrane protein TENB2; transmembrane protein with EGF-like and two follistatin-like domains 2; Transmembrane protein with EGF-like and two follistatin-like domains; TRtomoregulin-2 TMEFF2 CT120.2, HPP1, TENB2, TPEF, TR, TR-2 transmembrane protein with EGF like and two follistatin like domains 2 tomoregulin-2|cancer/testis antigen family 120, member 2|hyperplastic polyposis protein 1|transmembrane protein TENB2|transmembrane protein with EGF-like and two follistatin-like domains
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on TMEFF2, check out the TMEFF2 Infographic
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In this infographic, you will see the following information for TMEFF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For TMEFF2 (NM_016192) Human Over-expression Lysate (LS023671)
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No publications found for LS023671
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