USP13 (NM_003940) Human Over-expression Lysate

Product Name

USP13 (NM_003940) Human Over-expression Lysate

See all USP13 products

SKU/Catalog Number

LS008975

Size

100 µg

Description

Transient overexpression lysate of ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13)

Storage & Handling

Cite This Product

USP13 (NM_003940) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS008975)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for USP13 (Source: Uniprot.org, NCBI)

Alternative Names

Deubiquitinating enzyme 13; EC 3.1.2.15; EC 3.4.19.12; Isopeptidase T-3; IsoT-3; ISOT3IsoT-3; ubiquitin carboxyl-terminal hydrolase 13; ubiquitin specific peptidase 13 (isopeptidase T-3); ubiquitin specific protease 13 (isopeptidase T-3); Ubiquitin Thioesterase 13; Ubiquitin thiolesterase 13; Ubiquitin-specific-processing protease 13; USP13 USP13 ISOT3, IsoT-3 ubiquitin specific peptidase 13 ubiquitin carboxyl-terminal hydrolase 13|deubiquitinating enzyme 13|ubiquitin specific peptidase 13 (isopeptidase T-3)|ubiquitin specific protease 13 (isopeptidase T-3)|ubiquitin thioesterase 13|ubiquitin thiolesterase 13|ubiquitin-specific-processing protease 13

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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