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Product Info Summary
| SKU: | LS081030 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of Z-DNA binding protein 1 (ZBP1), transcript variant 1 |
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Product info
Product Name
Z DNA binding protein (ZBP1) (NM_030776) Human Over-expression Lysate
See all ZBP1/DLM-1/DAI products
SKU/Catalog Number
LS081030
Size
100 µg
Description
Transient overexpression lysate of Z-DNA binding protein 1 (ZBP1), transcript variant 1
Storage & Handling
Cite This Product
Z DNA binding protein (ZBP1) (NM_030776) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS081030)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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Z DNA binding protein (ZBP1) Human Over-expression Lysates NM_001160418.
Western blot validation of overexpression lysate (LS081030) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ZBP1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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Z DNA binding protein (ZBP1) Human Over-expression Lysates NM_030776.
Western blot validation of overexpression lysate (LS081030) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ZBP1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for Zbp1 (Source: Uniprot.org, NCBI)
Gene Name
Zbp1
Full Name
Z-DNA-binding protein 1
Weight
44.331kDa
Alternative Names
C20orf183; chromosome 20 open reading frame 183; DAI; DLM1; DLM-1; DLM1dJ718J7.3; DNA-dependent activator of interferon regulatory factors; DNA-dependent activator of IRFs; Tumor stroma and activated macrophage protein DLM-1; ZBP1 Z-DNA binding protein 1; ZBP1; Z-DNA binding protein 1; Z-DNA-binding protein 1 Zbp1|2010010H03Rik, D, Dai, Dlm1, mZaD, mZaDLM|Z-DNA binding protein 1|Z-DNA-binding protein 1|DNA-dependent activator of IFN-regulatory factors|tumor stroma and activated macrophage protein DLM-1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on Zbp1, check out the Zbp1 Infographic
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In this infographic, you will see the following information for Zbp1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Z DNA binding protein (ZBP1) (NM_030776) Human Over-expression Lysate (LS081030)
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