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Product Info Summary
| SKU: | EK7123 |
|---|---|
| Size: | 96 wells/kit |
| Reactive Species: | Human |
| Host: | Goat |
| Application: | ELISA |
| Sample Types: | See datasheet for sample type details |
Product info
Product Name
8-Isoprostane ELISA kit
SKU/Catalog Number
EK7123
Size
96 wells/kit
*Question: How many samples can I assay/run in this kit?
Description
This competitive ELISA kit is for determination of 8-isoprostane levels in biological samples. The isoprostanes are a family of eicosanoids of non-enzymatic origin produced by the random oxidation of tissue phospholipids by oxygen radicals. Each kit is for triplicate analyses of up to 24 samples and contains one 96 well plate, 8-isoprostane standard, 8-isoprostane-conjugated horseradish peroxidase (HRP), and buffers for sample and HRP dilutions, and plate washing.
Storage & Handling
All components are stored below -20°C and should not be re-frozen and thawed more than necessary. (Ship on dry ice)
Cite This Product
8-Isoprostane ELISA kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7123)
Host
Goat
Clonality of Antibodies
See Datasheet for details
Isotype
IgG
Sensitivity
10pg/ml
Assay Range
10-5000pg/ml
Reactive Species
EK7123 is reactive to in Human samples
Validated Sample Types
See datasheet for sample type details
Application Guarantee
EK7123 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
See datasheet for kit components details*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Data Example Images
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Plate Setup: Each plate must contain a minimum of three blank wells (BL), three maximum binding wells (BO), and a six point standard curve (S1-S6). Each sample should be assayed in triplicate. A suggested plate format is shown:
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The data shown here is an example of typical results obtained using 8-isoprostane ELISA kit. These results are only a guideline, and should not be used to determine values from your samples. The user must run their own standard curve every time.
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