Activin-B Human Recombinant Protein, Active

Activin B/Inhibin beta B protein, Human

Activin B human Recombinant produced in Nicotiana benthamiana plant is a beta-B single chain (aa 293-406) containing 123 amino acids (molecular formula C615H910N178O177S12). Activin B is fused to a 10-His-tag at the N-terminal having the total molecular mass of 14kDa and purified by standard chromatographic techniques.

Product Info Summary

SKU: PROTP09529
Size: 2ug, 10ug, 1mg
Origin Species: Human
Source: Nicotiana benthamiana plant

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Product Name

Activin-B Human Recombinant Protein, Active

View all Activin B/Inhibin beta B recombinant proteins

SKU/Catalog Number

PROTP09529

Size

2ug, 10ug, 1mg

Description

Activin B human Recombinant produced in Nicotiana benthamiana plant is a beta-B single chain (aa 293-406) containing 123 amino acids (molecular formula C615H910N178O177S12). Activin B is fused to a 10-His-tag at the N-terminal having the total molecular mass of 14kDa and purified by standard chromatographic techniques.

Storage & Handling

Lyophilized Activin B although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Activin B should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Cite This Product

Activin-B Human Recombinant Protein, Active (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP09529)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from 1mg/ml solution in 0.05M Tris-HCl buffer pH 7.4.

Purity

Greater than 97.0% as determined by Analysis by SDS-PAGE.

Predicted MW

45.122kDa

Reconstitution

It is recommended to reconstitute the lyophilized Activin B in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Amino Acid Sequence

GLECDGRTNL CCRQQFFIDF RLIGWNDWII APTGYYGNYC EGSCPAYLAG VPGSASSFHT AVVNQYRMRG LNPGTVNSCC IPTKLSTMSM LYFDDEYNIV KRDVPNMIVE ECG

Biological Activity

The biological activity of Activin B is measured by its ability to inhibit mouse plasmacytoma cell line (MPC-11) cells proliferation. EC50 less than 5ng/ml is required to stimulate a half-maximal response at cytokine saturation. Note: Since applications vary, each investigator should titrate the reagent to obtain optimal results.

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Reconstitution

It is recommended to reconstitute the lyophilized Activin B in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For INHBB (Source: Uniprot.org, NCBI)

Gene Name

INHBB

Full Name

Inhibin beta B chain

Weight

45.122kDa

Superfamily

TGF-beta family

Alternative Names

activin AB beta polypeptide; Activin B; Activin beta-B chain; INHBB; inhibin beta B chain; inhibin, beta B (activin AB beta polypeptide); inhibin, beta B; Inhibin, beta-2 INHBB inhibin subunit beta B inhibin beta B chain|Inhibin, beta-2|activin AB beta polypeptide|activin beta-B chain|inhibin beta B subunit

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on INHBB, check out the INHBB Infographic

INHBB infographic

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In this infographic, you will see the following information for INHBB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].

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