Product Info Summary
SKU: | LS009590 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of A kinase (PRKA) anchor protein 12 (AKAP12), transcript variant 1 |
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Product info
Product Name
AKAP12 (NM_005100) Human Over-expression Lysate
SKU/Catalog Number
LS009590
Size
100 µg
Description
Transient overexpression lysate of A kinase (PRKA) anchor protein 12 (AKAP12), transcript variant 1
Storage & Handling
Cite This Product
AKAP12 (NM_005100) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS009590)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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AKAP12 Human Over-expression Lysates NM_005100.
Western blot validation of overexpression lysate (LS009590) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with AKAP12 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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AKAP12 Human Over-expression Lysates NM_144497.
Western blot validation of overexpression lysate (LS009590) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with AKAP12 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for AKAP12 (Source: Uniprot.org, NCBI)
Gene Name
AKAP12
Full Name
A-kinase anchor protein 12
Weight
191.482kDa
Alternative Names
A kinase (PRKA) anchor protein (gravin) 12; A kinase (PRKA) anchor protein 12; AKAP 250; AKAP250250kDa; DKFZp686M0430; DKFZp686O0331; FLJ20945; FLJ97621; Gravin; kinase scaffold protein gravin; myasthenia gravis autoantigen gravin; Myasthenia gravis autoantigen AKAP12 AKAP250, SSeCKS A-kinase anchoring protein 12 A-kinase anchor protein 12|A kinase (PRKA) anchor protein 12|A-kinase anchor protein, 250kDa|AKAP 250|Src-Suppressed C Kinase Substrate|kinase scaffold protein gravin|myasthenia gravis autoantigen gravin
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on AKAP12, check out the AKAP12 Infographic
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In this infographic, you will see the following information for AKAP12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For AKAP12 (NM_005100) Human Over-expression Lysate (LS009590)
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