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Product Info Summary
| SKU: | LS056052 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae) (ALG1) |
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Product info
Product Name
ALG1 (NM_019109) Human Over-expression Lysate
SKU/Catalog Number
LS056052
Size
100 µg
Description
Transient overexpression lysate of asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae) (ALG1)
Storage & Handling
Cite This Product
ALG1 (NM_019109) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS056052)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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ALG1 Human Over-expression Lysates NM_019109.
Western blot validation of overexpression lysate (LS056052) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ALG1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for ALG1 (Source: Uniprot.org, NCBI)
Gene Name
ALG1
Full Name
Chitobiosyldiphosphodolichol beta-mannosyltransferase
Weight
52.518kDa
Superfamily
glycosyltransferase group 1 family
Alternative Names
asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase); asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S.cerevisiae); Asparagine-linked glycosylation protein 1 homolog; beta-1,4 mannosyltransferase; beta-1,4-mannosyltransferase; chitobiosyldiphosphodolichol beta-mannosyltransferase; GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase; GDP-mannose-dolichol diphosphochitobiose mannosyltransferase; hMat-1; HMAT1EC 2.4.1.142; HMT-1; HMT1CDG1K; mannosyltransferase-1; MT-1 ALG1 CDG1K, HMAT1, HMT-1, HMT1, MT-1, Mat-1, hMat-1 ALG1 chitobiosyldiphosphodolichol beta-mannosyltransferase chitobiosyldiphosphodolichol beta-mannosyltransferase|GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase|GDP-mannose-dolichol diphosphochitobiose mannosyltransferase|asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase)|asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog|asparagine-linked glycosylation protein 1 homolog|beta-1,4-mannosyltransferase|mannosyltransferase-1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on ALG1, check out the ALG1 Infographic
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In this infographic, you will see the following information for ALG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For ALG1 (NM_019109) Human Over-expression Lysate (LS056052)
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