Product Info Summary
SKU: | M14495-6 |
---|---|
Size: | 100ug |
Reactive Species: | Human, Vertebrates |
Host: | Rabbit |
Application: | ELISA, ICC, WB, Luminex |
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Product info
Product Name
Anti-Acetyl-Histone H4 (Lys5) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM199
SKU/Catalog Number
M14495-6
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Acetyl-Histone H4 (Lys5) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM199 (Catalog # M14495-6). Tested in WB, ICC, ELISA, Luminex applications. This antibody reacts with Human, Vertebrates.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Acetyl-Histone H4 (Lys5) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM199 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M14495-6)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM199
Isotype
Rabbit IgG
Immunogen
An acetyl-peptide corresponding to the Acetyl-Histone H4 (Lys5).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to Histone H4 acetylated at Lysine 5 (K5ac). No cross-reactivity with other acetylated Lysines in Histone H4.
Reactive Species
M14495-6 is reactive to H4C1 in Human, Vertebrates
Applications
M14495-6 is guaranteed for ELISA, ICC, WB, Luminex Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
11.367kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.2 ug/mL - 1 ug/mL
ICC: 0.5 ug/mL - 2 ug/mL
ELISA: 0.2 ug/mL - 1 ug/mL
Luminex: 0.05 ug/mL – 0.2 ug/Ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western Blotting result
Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate, and (2) recombinant Histone H4. Using RM199 at 0.2 ug/mL, showed a band of Histone H4 acetylated at Lysine 5 in HeLa cells.
Click image to see more details
Figure 2. Specificity Test result
RM199 specifically reacts to Histone H4 acetylated at Lysine 5 (K5ac). No cross reactivity with unmodified Lysine 5 (K5 ctrl), acetylated Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 16 (K16ac), Lysine 20 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.
Click image to see more details
Figure 3. ICC result
Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H4 (Lys5) Rabbit Monoclonal Antibody (Clone RM199) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Protein Target Info & Infographic
Gene/Protein Information For H4C1 (Source: Uniprot.org, NCBI)
Gene Name
H4C1
Full Name
Histone H4
Weight
11.367kDa
Superfamily
histone H4 family
Alternative Names
Histone H4 H4C1 H4-161, H4C12, H4C13, H4C14, H4C15, H4C2, H4C3, H4C4, H4C5, H4C6, H4C8, H4C9, H4FA, HIST1H4A, H4C1 H4 clustered histone 1 histone H4|H4 histone family, member A|histone 1, H4a|histone cluster 1 H4 family member a|histone cluster 1, H4a
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on H4C1, check out the H4C1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for H4C1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Acetyl-Histone H4 (Lys5) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM199 (M14495-6)
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No publications found for M14495-6
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