Anti-ADAMTS9 Antibody Picoband™

Product Name

Anti-ADAMTS9 Antibody Picoband™

View all ADAMTS9 Antibodies

SKU/Catalog Number

A04639-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ADAMTS9 Antibody Picoband™ catalog # A04639-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-ADAMTS9 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04639-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human ADAMTS9 recombinant protein (Position: F288-H1853).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A04639-1 is reactive to ADAMTS9 in Human, Rat

Applications

A04639-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

216 kDa

Calculated molecular weight

216.491kDa

Background of ADAMTS9

A disintegrin and metalloproteinase with thrombospondin motifs 9 is an enzyme that in humans is encoded by the ADAMTS9 gene. This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. Members of the ADAMTS family have been implicated in the cleavage of proteoglycans, the control of organ shape during development, and the inhibition of angiogenesis. This gene is localized to chromosome 3p14.3-p14.2, an area known to be lost in hereditary renal tumors. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Rat
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of ADAMTS9 using anti-ADAMTS9 antibody (A04639-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: rat H9C2(2-1) whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAMTS9 antigen affinity purified polyclonal antibody (Catalog # A04639-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAMTS9 at approximately 216 kDa. The expected band size for ADAMTS9 is at 216 kDa.

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Figure 2. Flow Cytometry analysis of THP-1 cells using anti-ADAMTS9 antibody (A04639-1).
Overlay histogram showing THP-1 cells stained with A04639-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADAMTS9 Antibody (A04639-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Figure 3. Flow Cytometry analysis of U20S cells using anti-ADAMTS9 antibody (A04639-1).
Overlay histogram showing U20S cells stained with A04639-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADAMTS9 Antibody (A04639-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For ADAMTS9 (Source: Uniprot.org, NCBI)

Alternative Names

ADAM metallopeptidase with thrombospondin type 1 motif, 9; ADAMTS-9,9; FLJ42955 ADAMTS9 ADAM metallopeptidase with thrombospondin type 1 motif 9 A disintegrin and metalloproteinase with thrombospondin motifs 9|a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 9

For more info on ADAMTS9, check out the ADAMTS9 Infographic

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In this infographic, you will see the following information for ADAMTS9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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