Product Info Summary
SKU: | M01263-1 |
---|---|
Size: | 100ul |
Reactive Species: | Bovine, Equine, Human, Mouse, Pig, Rat |
Host: | Mouse |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-All Six Isotypes Of Mammalian Actin Monoclonal Antibody
SKU/Catalog Number
M01263-1
Size
100ul
Description
Boster Bio Anti-All Six Isotypes Of Mammalian Actin Monoclonal Antibody catalog # M01263-1. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Equine, Human, Mouse, Pig, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-All Six Isotypes Of Mammalian Actin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01263-1)
Host
Mouse
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Monoclonal
Immunogen
Actin preparation from bovine brain
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01263-1 is reactive to in Bovine, Equine, Human, Mouse, Pig, Rat
Applications
M01263-1 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
41733 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000. IF/ICC or IHC: 1:500-1,000.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of tissue and cell lysates probed with mouse mAb against actin, M01263-1, in red. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The same blot was simultaneously probed with Boster chicken pAb to UCHL-1 , a marker of neuronal lineage cells, in green.
Click image to see more details
Immunofluorescent analysis of HeLa cells stained with mAb against actin, M01263-1, in green, costained with Boster chicken pAb against vimentin, in red. The actin antibody labels the submembranous actin-rich cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites. The vimentin antibody stains a different cytoskeletal network, the intermediate or 10nm filaments. The blue is DAPI staining of nuclear DNA.
Specific Publications For Anti-All Six Isotypes Of Mammalian Actin Monoclonal Antibody (M01263-1)
Hello CJ!
M01263-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
GSK-J4 induces cell cycle arrest and apoptosis via ER stress and the synergism between GSK-J4 and decitabine in acute myeloid leukemia KG-1a cells
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Customer Reviews
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