Human uPAR / URKR / uPA Receptor ELISA Kit PicoKine™

Human uPAR / URKR / uPA Receptor ELISA Kit PicoKine™ (96 Tests). Quantitate Human PLAUR in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 4pg/ml.

Product Info Summary

SKU: EK0536
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Product Name

Human uPAR / URKR / uPA Receptor ELISA Kit PicoKine™

See all uPAR products

SKU/Catalog Number

EK0536

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human uPAR / URKR / uPA Receptor ELISA Kit PicoKine™ (96 Tests). Quantitate Human PLAUR in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 4pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human uPAR / URKR / uPA Receptor ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0536)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: L23-R303

Sensitivity

<4 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0536 is reactive to PLAUR in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Application Guarantee

EK0536 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0536-CAP Anti-Human PLAUR Pre-coated 96-well strip microplate 1
EK0536-ST Human PLAUR Standard 2 vials, 10 ng/tube
EK0536-DA Human PLAUR Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0250.0850.1640.3450.6021.0781.5362.011

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human uPAR / URKR / uPA Receptor ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)16248713791464781361
Standard deviation10.6937.4966.1912.1137.2885.74
CV (%)6.6%7.7%4.8%8.3%7.8%6.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of uPAR in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11621741581681656.063.6%
Sample 248751446650949419.093.8%
Sample 31379123213751306132360.024.5%
*number of samples for each test n=16.

Gene/Protein Information For PLAUR (Source: Uniprot.Org, NCBI)

Uniprot ID

Q03405

Gene ID

5329

Gene Name

PLAUR

Full Name

Urokinase plasminogen activator surface receptor

Weight

36.978kDa

Alternative Names

CD87 antigen; CD87; Monocyte activation antigen Mo3; plasminogen activator, urokinase receptor; PLAUR; uPAR; U-PAR; UPARurokinase plasminogen activator surface receptor; u-plasminogen activator receptor form 2; URKRMO3 PLAUR CD87, U-PAR, UPAR, URKR plasminogen activator, urokinase receptor urokinase plasminogen activator surface receptor|monocyte activation antigen Mo3|u-plasminogen activator receptor form 2|urokinase-type plasminogen activator (uPA) receptor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PLAUR, check out the PLAUR Infographic

PLAUR infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PLAUR: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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5 Customer Q&As for Human uPAR / URKR / uPA Receptor ELISA Kit PicoKine™

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification uPAR using the EK0536 Human uPAR Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-02-20

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2020-02-20

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-03-05

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-03-05

Question

Q: is it okay to use citrate plasma as samples in Human uPAR Picokine® ELISA Kit (Catalog # EK0536)?

Verified Customer

Verified customer

Asked: 2018-05-12

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of uPAR causing disruption of its protein structure. uPAR may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the uPAR ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-05-12

Question

Q: how to proceed with the analysis of ELISA data? I have performed uPAR level in plasma.

H. Zhang

Verified customer

Asked: 2018-05-01

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-05-01

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

M. Mehta

Verified customer

Asked: 2014-10-19

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2014-10-19

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