Anti-Anillin/ANLN Antibody Picoband™

Product Name

Anti-Anillin/ANLN Antibody Picoband™

View all Anillin Antibodies

SKU/Catalog Number

A03997-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Anillin/ANLN Antibody Picoband™ catalog # A03997-1. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Anillin/ANLN Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03997-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Anillin/ANLN, which shares 96.4% amino acid (aa) sequence identity with both mouse and rat Anillin/ANLN.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03997-1 is reactive to ANLN in Human

Applications

A03997-1 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

150-160 kDa

Calculated molecular weight

124.199kDa

Background of Anillin

Anillin is a conserved protein implicated in cytoskeletal dynamics during cellularization and cytokinesis. This gene is mapped to 7p14.2. The ANLN gene in humans and the scraps gene in Drosophila encode Anillin. The human anillin cDNA, located on Chr7, encodes a 1,125–amino acid protein with a predicted molecular mass of 124 kD and a pI of 8.1. The mouse anillin gene is located on Chr9. This gene encodes an actin-binding protein that plays a role in cell growth and migration, and in cytokinesis. The encoded protein is thought to regulate actin cytoskeletal dynamics in podocytes, components of the glomerulus. Mutations in this gene are associated with focal segmental glomerulosclerosis 8. Alternative splicing results in multiple transcript variants encoding different isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 5 μg/ml
Flow Cytometry, 1-3μg/1x10⁶ cells

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Anillin/ANLN using anti-Anillin/ANLN antibody (A03997-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Anillin/ANLN antigen affinity purified polyclonal antibody (Catalog # A03997-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Anillin/ANLN at approximately 150-160 kDa. The expected band size for Anillin/ANLN is at 123 kDa.

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Figure 2. IF analysis of Anillin/ANLN using anti-Anillin/ANLN antibody (A03997-1).
Anillin/ANLN was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Anillin/ANLN Antibody (A03997-1) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of U20S cells using anti-Anillin/ANLN antibody (A03997-1).
Overlay histogram showing U20S cells stained with A03997-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Anillin/ANLN Antibody (A03997-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For ANLN (Source: Uniprot.org, NCBI)

Alternative Names

actin-binding protein anillin; anillin (Drosophila Scraps homolog), actin binding protein; ANILLIN; anillin, actin binding protein (scraps homolog, Drosophila); anillin, actin binding protein; DKFZp779A055; Drosophila Scraps homolog; scra; Scraps ANLN FSGS8, Scraps, scra anillin actin binding protein anillin

For more info on ANLN, check out the ANLN Infographic

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In this infographic, you will see the following information for ANLN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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