Anti-AP-1 complex subunit gamma-1 AP1G1 Antibody

Product Name

Anti-AP-1 complex subunit gamma-1 AP1G1 Antibody

View all Gamma Adaptin Antibodies

SKU/Catalog Number

A12998

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-AP-1 complex subunit gamma-1 AP1G1 Antibody (Catalog # A12998). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-AP-1 complex subunit gamma-1 AP1G1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12998)

Host

Goat

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 625-675 of Human AP1G1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A12998 is reactive to AP1G1 in Human

Applications

A12998 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

91.351kDa

Background of Gamma Adaptin

AP1G1 (also known as gamma 1 subunit of adaptor -related protein complex 1, Gamma-adaptin, Adaptor protein complex AP-1 gamma-1 subunit, Golgi adaptor HA1/AP1 adaptin gamma-1 subunit and Clathrin assembly protein complex 1 gamma-1 large chain) is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. AP1G1 is localized to the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex and is widely expressed.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,000 - 1:8,000
IP: 1:100
WB: 1:500 - 1:2,000

Validation Images & Assay Conditions

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Western blot analysis of AP1G1 expression in HELA whole cell lysates (lane 1), Peptide competition (using 1

Gene/Protein Information For AP1G1 (Source: Uniprot.org, NCBI)

Alternative Names

Adapter-related protein complex 1 subunit gamma-1; Adaptor protein complex AP-1 subunit gamma-1; adaptor-related protein complex 1, gamma 1 subunit; ADTGclathrin assembly protein complex 1 gamma large chain; AP-1 complex subunit gamma-1; CLAPG1clathrin-associated/assembly/adaptor protein, large, gamma 1; Clathrin assembly protein complex 1 gamma-1 large chain; gamma adaptin; gamma1-adaptin; golgi adaptor HA1/AP1 adaptin gamma subunit; Golgi adaptor HA1/AP1 adaptin subunit gamma-1; MGC18255 AP1G1 ADTG, CLAPG1 adaptor related protein complex 1 subunit gamma 1 AP-1 complex subunit gamma-1|adapter-related protein complex 1 subunit gamma-1|adaptor protein complex AP-1 subunit gamma-1|adaptor related protein complex 1 gamma 1 subunit|clathrin assembly protein complex 1 gamma large chain|clathrin assembly protein complex 1 gamma-1 large chain|clathrin-associated/assembly/adaptor protein, large, gamma 1|gamma adaptin|gamma1-adaptin|golgi adaptor HA1/AP1 adaptin gamma subunit|golgi adaptor HA1/AP1 adaptin subunit gamma-1|testicular tissue protein Li 21

For more info on AP1G1, check out the AP1G1 Infographic

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In this infographic, you will see the following information for AP1G1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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