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Product Info Summary
| SKU: | A01497 |
|---|---|
| Size: | 1mg |
| Reactive Species: | Human |
| Host: | Goat |
| Application: | ELISA, IP, IHC, WB |
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Product info
Product Name
Anti-Apolipoprotein C-II APOC2 Antibody
SKU/Catalog Number
A01497
Size
1mg
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Apolipoprotein C-II APOC2 Antibody (Catalog # A01497). Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store vial at 4°C prior to opening. This product is stable 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening. (Ship on wet ice.)
Cite This Product
Anti-Apolipoprotein C-II APOC2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01497)
Host
Goat
Contents
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
apoLipoprotein Type C-II produced synthetically in full-length form (not selected epitopes) using conventional peptide technology.
Reactive Species
A01497 is reactive to APOC2 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
11.3 kDa
Background of APOC2
This antibody is suitable for cardiovascular research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01497 is guaranteed for ELISA, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:10,000
IHC: 1:50 - 1:200
IP: 1:100
WB: 1:5,000 - 1:10,000
Anti-apoLipoprotein antibodies have been tested in immunohistochemistry and used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, and for western blotting for highly sensitive qualitative analysis.
Validation Images & Assay Conditions
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Immunohistochemistry of goat anti-Apolipoprotein C-II antibody. Tissue: human kidney. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Apolipoprotein C-II at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase goat secondary antibody at 1:10,000 for 45 min at RT. Staining: Apolipoprotein C-II as precipitated red signal with hematoxylin purple nuclear counterstain.
Specific Publications For Anti-Apolipoprotein C-II APOC2 Antibody (A01497)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Apolipoprotein C-II APOC2 Antibody
Question
Would A01497 anti-Apolipoprotein C-II antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
As indicated on the product datasheet, A01497 anti-Apolipoprotein C-II antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-10
Question
Do you have a BSA free version of anti-Apolipoprotein C-II antibody A01497 available?
S. Jones
Verified customer
Asked: 2019-11-15
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Apolipoprotein C-II antibody A01497 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-15
Question
I see that the anti-Apolipoprotein C-II antibody A01497 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-06-16
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-06-16
Question
I have attached the WB image, lot number and protocol we used for skeletal muscle using anti-Apolipoprotein C-II antibody A01497. Please let me know if you require anything else.
A. Parker
Verified customer
Asked: 2015-04-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-04-08
Question
We are currently using anti-Apolipoprotein C-II antibody A01497 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
C. Krishna
Verified customer
Asked: 2014-03-21
Answer
The anti-Apolipoprotein C-II antibody (A01497) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-03-21
