Anti-ATRX / RAD54 (Alpha Thalassemia Mental Retardation) Monoclonal Antibody

Product Name

Anti-ATRX / RAD54 (Alpha Thalassemia Mental Retardation) Monoclonal Antibody

View all ATRX Antibodies

SKU/Catalog Number

M00203

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-ATRX / RAD54 (Alpha Thalassemia Mental Retardation) Monoclonal Antibody (Catalog # M00203). Tested in IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-ATRX / RAD54 (Alpha Thalassemia Mental Retardation) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00203)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: 39f

Isotype

IgG1, kappa

Immunogen

Recombinant full-length human ATRX protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00203 is reactive to ATRX in Human

Applications

M00203 is guaranteed for IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

282.586kDa

Background of ATRX

ATRX is a member of the Snf2 family of helicase/ATPases, which contribute to the remodeling of the nucelosome structure in an ATP-dependent manner, and facilitate the initiation of transcription and replication. Structurally, ATRX contains a PHD zinc finger motif. ATRX is regulated throughout the cell cycle where it is differentially distributed within the nucleus. During interphase, ATRX predominately associates with the nuclear matrix, while during mitosis, ATRX localizes with condensed chromatin. At the onset of M phase, phosphorylation rapidly induces this redistribution of ATRX to the short arms of human acrocentric chromosomes, where it then specifically complexes with heterochromatin protein 1 thalassemia or ATRX syndrome

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Pancreas stained with Anti-ATRX Mouse Monoclonal Antibody (39f).

Gene/Protein Information For ATRX (Source: Uniprot.org, NCBI)

Alternative Names

alpha thalassemia/mental retardation syndrome X-linked (RAD54 (S. cerevisiae)homolog); alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S.cerevisiae); alpha thalassemia/mental retardation syndrome X-linked; ATP-dependent helicase ATRX; ATR2; DNA dependent ATPase and helicase; EC 3.6.1; EC 3.6.4.12; helicase 2, X-linked; Juberg-Marsidi syndrome; MGC2094; MRXHF1; RAD54 homolog; RAD54L; SFM1; SHS; transcriptional regulator ATRX; XH2RAD54; X-linked helicase II; X-linked nuclear protein; XNPZNF-HX; Zinc finger helicase; Znf-HX ATRX JMS, MRX52, RAD54, RAD54L, XH2, XNP, ZNF-HX ATRX chromatin remodeler transcriptional regulator ATRX|ATP-dependent helicase ATRX|X-linked helicase II|X-linked nuclear protein|alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S. cerevisiae)

For more info on ATRX, check out the ATRX Infographic

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In this infographic, you will see the following information for ATRX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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