Product Info Summary
SKU: | M00246-4 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Aurora A Kinase Monoclonal Antibody
SKU/Catalog Number
M00246-4
Size
100ul
Description
Boster Bio Anti-Aurora A Kinase Monoclonal Antibody catalog # M00246-4. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Aurora A Kinase Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00246-4)
Host
Mouse
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Monoclonal
Immunogen
Full length recombinant human aurora A expressed in and purified from E. coli
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00246-4 is reactive to in Human, Mouse, Rat
Applications
M00246-4 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
46372 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:100-1:500. ICC/IF and IHC: 1:100-1:500.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of different cell lysates and recombinant protein solutions using mouse mAb to aurora A, M00246-4. Left: cells were treated with 100ng/mL of nocodazol (a microtubule depolymerizing agent which induces cells to halt at G2/M phase) for 6 hours: [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] Equine NBL6 cells, and [5] murine KR158 cells. The antibody apparently does not recognize canine or equine aurora A, but does bind human and murine. Right: equal amounts of purified human recombinant aurora A, B, C as indicated. The M00246-4 antibody reacts with the aurora A recombinant protein but not aurora B or C.
Click image to see more details
HeLa cell cultures stained with M00246-4 antibody in green. Aurora A localizes in spindle poles and mitotic spindles at late mitosis. The cells are counterstained with Boster chicken polyclonal antibody to vimentin,
Specific Publications For Anti-Aurora A Kinase Monoclonal Antibody (M00246-4)
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