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Product Info Summary
| SKU: | A13589 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-BCAS1 Antibody
SKU/Catalog Number
A13589
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-BCAS1 Antibody (Catalog # A13589). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-BCAS1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13589)
Host
Rabbit
Contents
BCAS1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
BCAS1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human BCAS1. The immunogen is located within amino acids 460 - 510 of BCAS1.
Reactive Species
A13589 is reactive to BCAS1 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
61.7 kDa
Background of BCAS1
BCAS1 was identified through positional cloning and was found to be overexpressed in most but not all breast cancer cells lines. Little is known about this protein; BCAS1 is cytoplasmically localized and is thought to form homodimers through coiled-coil structures. High levels of BCAS1 mRNA are seen in brain and prostate tissues, with lower amounts observed in colon, intestine and testis. Cells engineered to overexpress BCAS1 did not lose anchorage-dependent growth or increase their rate of growth, suggesting that BCAS1 is not a prototypical oncogene. The BCAS1 gene was also found to be amplified in other carcinomas such as pancreatic carcinoma, suggesting that BCAS1 may play in important role in the control of cell proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13589 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
BCAS1 antibody can be used for detection of BCAS1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of BCAS1 in human lung tissue lysate with BCAS1 antibody at 1 μg/mL.
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Immunohistochemistry of BCAS1 in human breast carcinoma with BCAS1 antibody at 5 μg/mL.
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Immunofluorescence of BCAS1 in Human Breast Carcinoma cells with BCAS1 antibody at 20 μg/mL.
Specific Publications For Anti-BCAS1 Antibody (A13589)
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