Anti-Bi-1 TMBIM6 Antibody (Monoclonal, ABM20E4)

Product Name

Anti-Bi-1 TMBIM6 Antibody (Monoclonal, ABM20E4)

View all TMBIM6 Antibodies

SKU/Catalog Number

M05462

Size

100 µg

Form

Liquid

Description

Boster Bio Anti-Bi-1 TMBIM6 Antibody (Monoclonal, ABM20E4) (Catalog# M05462). Tested in WB application(s). This antibody reacts with Human.

Storage & Handling

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Cite This Product

Anti-Bi-1 TMBIM6 Antibody (Monoclonal, ABM20E4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05462)

Host

Mouse

Contents

100 µg in 200 µl PBS containing 0.05% stabilizing protein and 0.05% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

ABM20E4

Isotype

IgG2a Kappa

Immunogen

A partial length recombinant protein of human Bi-1 (amino acids 1-200)

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M05462 is reactive to TMBIM6 in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

26.5 kDa

Background of TMBIM6

This monoclonal antibody recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M05462 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot: 4-6 µg/ml

Validation Images & Assay Conditions

See full target information TMBIM6

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