Product Info Summary
| SKU: | A15680 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-BIVM Antibody
SKU/Catalog Number
A15680
Size
100uL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-BIVM Antibody (Catalog # A15680). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-BIVM Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15680)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
BIVM peptide corresponding to a region near carboxy terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Reactive Species
A15680 is reactive to BIVM in Human
Calculated molecular weight
56.8 kDa
Background of BIVM
BIVM (for basic, immunoglobulin-like variable motif-containing) refers to a recently identified gene product that maps to human chromosome 13q32-q33 and is predicted to encode a 503-amino acid protein. BIVM shows ubiquitous expression in normal human tissue and the presence of a 5' CpG island suggests it is a housekeeping gene. BIVM is likely essential for some aspect of basic cellular function. BIVM is highly charged and localizes to the cytoplasm and nucleus where it may bind to either DNA or RNA or associate with other cellular proteins. Significant sequence homology exists with many organisms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15680 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:25,000
WB: 1:1,000
This polyclonal antibody reacts human BIVM at the C-terminal region and is useful for detection of BIVM by ELISA or western blotting. For immunoblotting a 57 kDa band corresponding to human BIVM is detected. Unstimulated HeLa cells can be used as a positive control. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.
Validation Images & Assay Conditions
Click image to see more details
Western blot of anti-human BIVM. Whole cell HeLa lysate was used to probe for endogenous BIVM using Rabbit-anti-Human BIVM (C-terminal specific) polyclonal antibody. A 57 kDa band corresponding to human BIVM protein is detected using a 1:1,000 dilution of the antiserum incubated for 1 hour at room temperature. Washes with 1% Tween-20 TBS preceded reaction with HRP Gt-a-Rabbit IgG (H&L) code 611-103-122 diluted 1:10,000 and incubated for 1 hour at room temperature. The blot was developed using a chemiluminescent detection method (AP ECL 60 sec followed by a 45 sec exposure). Other detection methods will yield similar results.
Specific Publications For Anti-BIVM Antibody (A15680)
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4 Customer Q&As for Anti-BIVM Antibody
Question
you antibody to test anti-BIVM antibody A15680 on human corpus callosum for research purposes, then I may be interested in using anti-BIVM antibody A15680 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
The products we sell, including anti-BIVM antibody A15680, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-12
Question
Can you help my question with product A15680, anti-BIVM antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A15680 anti-BIVM antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-11
Question
I was wanting to use your anti-BIVM antibody for WB for human corpus callosum on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human corpus callosum identification?
Verified Customer
Verified customer
Asked: 2018-10-30
Answer
As indicated on the product datasheet, A15680 anti-BIVM antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human corpus callosum in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-30
Question
We are currently using anti-BIVM antibody A15680 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
K. Singh
Verified customer
Asked: 2015-03-04
Answer
The anti-BIVM antibody (A15680) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-03-04

