Anti-Bradykinin Receptor B₁ Bdkrb1 Antibody

Product Name

Anti-Bradykinin Receptor B₁ Bdkrb1 Antibody

View all Bradykinin RB1/BDKRB1 Antibodies

SKU/Catalog Number

A05724

Size

100µg

Form

Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.

Description

Boster Bio Anti-Bradykinin Receptor B₁ Bdkrb1 Antibody catalog # A05724. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Bradykinin Receptor B₁ Bdkrb1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05724)

Host

Rabbit

Contents

Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

Synthetic peptide corresponding to the sequence near the N-terminus of rat bradykinin receptor B₁.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A05724 is reactive to BDKRB1 in Mouse, Rat

Applications

A05724 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

40.495kDa

Background of Bradykinin RB1/BDKRB1

SmarcAL1 (SWI/SNF-related µatrix-associated actin-dependent regulator of chromatin a-like 1) is also known as HARP, HepA Related Protein, HHARP, SMARCA like Protein 1 and SWI/SNF Related. SmarcAL1 is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

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Membrane ELISA: Lewis rat heart membranes (5µ

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Figure 1. Western blot analysis of Bdkrb1 using anti-Bdkrb1 antibody (A05724).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bdkrb1 antigen affinity purified polyclonal antibody (Catalog # A05724) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Bdkrb1.

Gene/Protein Information For BDKRB1 (Source: Uniprot.org, NCBI)

Alternative Names

B1 bradykinin receptor; B1BKR; B1R; BDKRB1; BK-1 Receptor; BKB1R; BKR1; BRADYB1; bradykinin B1 receptor; Bradykinin RB1; bradykinin receptor 1; bradykinin receptor B1; BradykininRB1 BDKRB1 B1BKR, B1R, BKB1R, BKR1, BRADYB1 bradykinin receptor B1 B1 bradykinin receptor|BK-1 receptor|bradykinin B1 receptor|bradykinin receptor 1

For more info on BDKRB1, check out the BDKRB1 Infographic

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In this infographic, you will see the following information for BDKRB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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