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Product Info Summary
| SKU: | A08646-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-BRAF35 HMG20B Antibody
SKU/Catalog Number
A08646-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-BRAF35 HMG20B Antibody catalog # A08646-2. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BRAF35 HMG20B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08646-2)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human BRAF35
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08646-2 is reactive to HMG20B in Human
Applications
A08646-2 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
35.813kDa
Background of BRAF35
Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Catalyzes the ISGylation of influenza A virus NS1 protein.
Sutisak Kitareewan, PNAS, Mar 2002; 99: 3806.
Keun Il Kim, Mol. Cell. Biol., Jan 2006; 26: 472 - 479.
Ian Pitha-Rowe, Cancer Res., Nov 2004; 64: 8109 - 8115.<
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Figure 1. Western blotting validation for Anti-BRAF35 HMG20B Antibody A08646-2
Western Blot (WB) analysis of specific cells using BRAF35 polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
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Figure 2. Western blotting validation for Anti-BRAF35 HMG20B Antibody A08646-2
Western Blot (WB) analysis of AD293 cells using BRAF35 polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For HMG20B (Source: Uniprot.org, NCBI)
Gene Name
HMG20B
Full Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related
Weight
35.813kDa
Alternative Names
BRAF35Sox-like transcriptional factor; high-mobility group 20B; HMG domain-containing protein 2; HMG domain-containing protein HMGX2; HMGX2FLJ26127; HMGXB2member 1-related; SMARCE1R; SMARCE1-related protein HMG20B BRAF25, BRAF35, HMGX2, HMGXB2, PP7706, SMARCE1r, SOXL, pp8857 high mobility group 20B SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related|BRCA2-associated factor 35|HMG box domain containing 2|HMG box-containing protein 20B|HMG domain-containing protein 2|HMG domain-containing protein HMGX2|SMARCE1-related protein|SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E, member 1-related|Sox-like transcriptional factor|structural DNA-binding protein BRAF35
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HMG20B, check out the HMG20B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HMG20B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BRAF35 HMG20B Antibody (A08646-2)
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No publications found for A08646-2
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