Product Info Summary
SKU: | A08420-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-BRD9 Antibody Picoband™
SKU/Catalog Number
A08420-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-BRD9 Antibody Picoband™ catalog # A08420-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-BRD9 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08420-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human BRD9 recombinant protein (Position: K7-K271).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08420-1 is reactive to BRD9 in Human, Mouse, Rat
Applications
A08420-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
80 kDa
Calculated molecular weight
67kDa
Background of BRD9
Bromodomain-containing protein 9 is a protein that in humans is encoded by the BRD9 gene. Enables lysine-acetylated histone binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Located in nucleoplasm. Part of SWI/SNF complex.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 μg/1x106 cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of BRD9 using anti-BRD9 antibody (A08420-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: human MOLT-4 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRD9 antigen affinity purified polyclonal antibody (Catalog # A08420-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRD9 at approximately 80 kDa. The expected band size for BRD9 is at 67 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of PC-3 cells using anti-BRD9 antibody (A08420-1).
Overlay histogram showing PC-3 cells stained with A08420-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BRD9 Antibody (A08420-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For BRD9 (Source: Uniprot.org, NCBI)
Gene Name
BRD9
Full Name
Bromodomain-containing protein 9
Weight
67kDa
Alternative Names
bromodomain containing 9; bromodomain-containing protein 9; DKFZp434D0711; DKFZp686L0539; FLJ13441; FLJ43530; LAVS3040; PRO9856; Rhabdomyosarcoma antigen MU-RMS-40.8; sarcoma antigen NY-SAR-29 BRD9 LAVS3040, PRO9856 bromodomain containing 9 bromodomain-containing protein 9|rhabdomyosarcoma antigen MU-RMS-40.8|sarcoma antigen NY-SAR-29
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BRD9, check out the BRD9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BRD9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BRD9 Antibody Picoband™ (A08420-1)
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