Anti-BSCL2/Seipin Antibody Picoband™

Product Name

Anti-BSCL2/Seipin Antibody Picoband™

View all Seipin/BSCL2 Antibodies

SKU/Catalog Number

A02753-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-BSCL2/Seipin Antibody Picoband™ catalog # A02753-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-BSCL2/Seipin Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02753-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human BSCL2/Seipin recombinant protein (Position: A87-P231).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02753-1 is reactive to Bscl2 in Human, Mouse, Rat

Applications

A02753-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

46 kDa

Calculated molecular weight

43.105kDa

Background of Seipin/BSCL2

Seipin is a protein that in humans is encoded by the BSCL2 gene. This gene encodes the multi-pass transmembrane protein protein seipin. This protein localizes to the endoplasmic reticulum and may be important for lipid droplet morphology. Mutations in this gene have been associated with congenital generalized lipodystrophy type 2 or Berardinelli-Seip syndrome, a rare autosomal recessive disease characterized by a near absence of adipose tissue and severe insulin resistance. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Naturally occurring read-through transcription occurs between this locus and the neighboring locus HNRNPUL2 (heterogeneous nuclear ribonucleoprotein U-like 2).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of BSCL2/Seipin using anti-BSCL2/Seipin antibody (A02753-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: human Hacat whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BSCL2/Seipin antigen affinity purified polyclonal antibody (Catalog # A02753-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BSCL2/Seipin at approximately 46 kDa. The expected band size for BSCL2/Seipin is at 44 kDa.

Gene/Protein Information For Bscl2 (Source: Uniprot.org, NCBI)

Alternative Names

Berardinelli-Seip congenital lipodystrophy 2 (seipin); Bernardinelli-Seip congenital lipodystrophy 2 (seipin); Bernardinelli-Seip congenital lipodystrophy type 2 protein; BSCL2; FLJ16651; GNG3LG; HMN5; MGC4694; Seipin; spastic paraplegia 17 (Silver syndrome); SPG17 Bscl2|2900097C17Rik, AI046355, Gng3, Gng3lg, s|Berardinelli-Seip congenital lipodystrophy 2 (seipin)|seipin|Berardinelli-Seip congenital lipodystrophy 2 homolog (seipin)|Bernardinelli-Seip congenital lipodystrophy 2 homolog|G protein gamma 3 linked|bernardinelli-Seip congenital lipodystrophy type 2 protein homolog

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