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Product Info Summary
| SKU: | A04257 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-BTN3A1/2/3 Antibody
View all CD277/BTN3A1 Antibodies
SKU/Catalog Number
A04257
Size
100μl
Form
Liquid
Description
Boster Bio Anti-BTN3A1/2/3 Antibody catalog # A04257. Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BTN3A1/2/3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04257)
Host
Rabbit
Contents
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Clonality
Polyclonal
Clone Number
5G7
Isotype
IgG
Immunogen
Synthetic peptide from human protein at AA range: 41-90.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04257 is reactive to BTN3A1 in Human
Applications
A04257 is guaranteed for IHC Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
57.677kDa
Background of CD277/BTN3A1
Cysteine string protein (CSP) is a 34 kDa rat brain protein localized to the cytoplasmic surface of the synaptic vesicle. CSP is believed to perform similar functions in the assembly of synaptic vesicle proteins which are essential to membrane trafficking.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC-P, 1:50-300
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200.
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Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200.
Protein Target Info & Infographic
Gene/Protein Information For BTN3A1 (Source: Uniprot.org, NCBI)
Gene Name
BTN3A1
Full Name
Butyrophilin subfamily 3 member A1
Weight
57.677kDa
Superfamily
immunoglobulin superfamily
Alternative Names
BT3.1; BTF5; BTN3A1; butyrophilin, subfamily 3, member A1; CD277; dJ45P21.3 (butyrophilin, subfamily 3, member A1) BTN3A1 BT3.1, BTF5, BTN3.1, CD277 butyrophilin subfamily 3 member A1 butyrophilin subfamily 3 member A1|dJ45P21.3 (butyrophilin, subfamily 3, member A1)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BTN3A1, check out the BTN3A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BTN3A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BTN3A1/2/3 Antibody (A04257)
Hello CJ!
No publications found for A04257
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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13 Customer Q&As for Anti-BTN3A1/2/3 Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for hippocampus thalamus using anti-BTN3A1/2/3 antibody A04257. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-07
Question
Does anti-BTN3A1/2/3 antibody A04257 work for IHC with hippocampus thalamus?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
According to the expression profile of hippocampus thalamus, BTN3A1 is highly expressed in hippocampus thalamus. So, it is likely that anti-BTN3A1/2/3 antibody A04257 will work for IHC with hippocampus thalamus.
Boster Scientific Support
Answered: 2020-02-27
Question
We are currently using anti-BTN3A1/2/3 antibody A04257 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
J. Miller
Verified customer
Asked: 2019-12-17
Answer
The anti-BTN3A1/2/3 antibody (A04257) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-17
Question
My lab would like to test anti-BTN3A1/2/3 antibody A04257 on human hippocampus thalamus for research purposes, then I may be interested in using anti-BTN3A1/2/3 antibody A04257 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
The products we sell, including anti-BTN3A1/2/3 antibody A04257, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-13
Question
I was wanting to use your anti-BTN3A1/2/3 antibody for IHC for human hippocampus thalamus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human hippocampus thalamus identification?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
It shows on the product datasheet, A04257 anti-BTN3A1/2/3 antibody has been tested for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human hippocampus thalamus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-28
Question
I see that the anti-BTN3A1/2/3 antibody A04257 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-10
Question
Would anti-BTN3A1/2/3 antibody A04257 work on goat IHC with small intestine peyer's patch?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
Our lab technicians have not tested anti-BTN3A1/2/3 antibody A04257 on goat. You can run a BLAST between goat and the immunogen sequence of anti-BTN3A1/2/3 antibody A04257 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat small intestine peyer's patch in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-31
Question
Is there a BSA free version of anti-BTN3A1/2/3 antibody A04257 available?
Verified Customer
Verified customer
Asked: 2019-01-23
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-BTN3A1/2/3 antibody A04257 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-01-23
Question
My question regarding product A04257, anti-BTN3A1/2/3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-12-07
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04257 anti-BTN3A1/2/3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-07
Question
Is this A04257 anti-BTN3A1/2/3 antibody reactive to the isotypes of BTN3A1?
H. Jones
Verified customer
Asked: 2017-06-21
Answer
The immunogen of A04257 anti-BTN3A1/2/3 antibody is Synthetic peptide from human protein at AA range: 41-90. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-06-21
Question
Is a blocking peptide available for product anti-BTN3A1/2/3 antibody (A04257)?
Verified Customer
Verified customer
Asked: 2017-06-21
Answer
We do provide the blocking peptide for product anti-BTN3A1/2/3 antibody (A04257). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-06-21
Question
Will A04257 anti-BTN3A1/2/3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-05-25
Answer
As indicated on the product datasheet, A04257 anti-BTN3A1/2/3 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-05-25
Question
Here is the WB image, lot number and protocol we used for hippocampus thalamus using anti-BTN3A1/2/3 antibody A04257. Please let me know if you require anything else.
S. Parker
Verified customer
Asked: 2014-04-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-04-14
