Rabbit IgG polyclonal antibody for Calcium/calmodulin-dependent protein kinase kinase 2(CAMKK2) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.
Anti-CAMKK2 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Applications:||IHC, ICC, WB|
|Product Name||Anti-CAMKK2 Antibody
See all CAMKK2 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for CAMKKBETA/CAMKK2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. CAMKKBETA/CAMKK2 information: Molecular Weight: 64746 MW; Subcellular Localization: Nucleus . Cytoplasm . Cell projection . Predominantly nuclear in unstimulated cells (By similarity). Found in the cytoplasm and neurites after forskolin induction; Tissue Specificity: Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung.|
|Cite This Product||Anti-CAMKK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1497)|
|Similar Products From
|Anti-CAMKK2 Antibody may replace the following items: sc 134086|sc 51232|sc 51227|sc 51231.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Applications||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human CAMKK2(180-194aa KLAYNENDNTYYAMK), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For CAMKK2 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||10645|
|Species Of This Entry||Human|
|Protein Name||Calcium/calmodulin-dependent protein kinase kinase 2|
|Superfamily||protein kinase superfamily|
|Alternative Names||CAMKK2|calcium/calmodulin-dependent protein kinase kinase 2; calcium/calmodulin-dependent protein kinase kinase 2, beta; Calcium/calmodulin-dependent protein kinase kinase beta; CaM-kinase kinase 2; CaM-kinase kinase beta; CaM-KK 2; CAMKK beta protein; CaMKK beta; CaM-KK beta; CAMKK; CAMKKBCaMKK 2; EC 2.7.11; EC 184.108.40.206; KIAA0787calcium/calmodulin-dependent protein kinase beta; MGC15254|
|Post Tranlational Modifications||Phosphorylation; Oxidation|
|Subcellular Localization||Nucleus. Cytoplasm. Cell projection, neuron projection. Predominantly nuclear in unstimulated cells, relocalizes into cytoplasm and neurites after forskolin induction.|
|Gene Location||12q24.31, on Chromasome 12, gene sequence: NC_000012.12 (121237692..121298308, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For CAMKK2 (Source: Uniprot.org, NCBI)
|Protein Function||Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin- binding domain are inactive. Efficiently phosphorylates 5'-AMP- activated protein kinase (AMPK) trimer, including that consisting of PRKAA1, PRKAB1 and PRKAG1. This phosphorylation is stimulated in response to Ca(2+) signals (By similarity). Seems to be involved in hippocampal activation of CREB1 (By similarity). May play a role in neurite growth. Isoform 3 may promote neurite elongation, while isoform 1 may promoter neurite branching.|
|Tissue Specificity||Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung.|
|Research Areas||Protein Kinase|
|Related Diseases||Malignant Neoplasms; Nervousness; Neoplasms; Impaired Glucose Tolerance; Inflammation; Obesity; Anxiety Disorders; Prostatic Neoplasms; Insulin Resistance; Malignant Neoplasm Of Prostate; Cholangiocarcinoma; Learning Disorders; Sclerosis; Malnutrition; Aneuploidy; Malignant Tumor Of Cervix; Severe Combined Immunodeficiency|
|Related Pathways||Glucose Homeostasis; Cell Proliferation; Localization; Rna Interference; Anaphase; Cell Cycle; Long-term Memory; Granulocyte Differentiation; Cell Migration; Brain Development; Macroautophagy; Mating; Estrus; Insulin Secretion; Cell Development; Mitosis; Energy Homeostasis; Dna Replication; Short-term Memory; Cell Death|
|Background||CAMKK2, Calcium/calmodulin-dependent protein kinase kinase 2, is an enzyme that in humans is encoded by the CAMKK2 gene. The product of this gene belongs to the serine/threonine-specific protein kinase family, and to the Ca++/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent(CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. CaMKK2 regulates production of the appetite stimulating hormone neuropeptide Y and functions as an AMPK kinase in the hypothalamus. It also has an important role in the development of hyperalgesia and tolerance to opioid analgesic drugs, through reduction in downstream signalling pathways and mu opioid receptor downregulation. Inhibition of CaMKK2 in mice reduces appetite and promotes weight loss.|
|Scientific References||PMID: 11395482 by Hsu L.-S., et al. Human Ca2+/calmodulin-dependent protein kinase kinase beta gene encodes multiple isoforms that display distinct kinase activity.|
PMID: 9822657 by Anderson K.A., et al. Components of a calmodulin-dependent protein kinase cascade. Molecular cloning, functional characterization and cellular localization of Ca2+/calmodulin-dependent protein kinase kinase beta.
Our Boster Quality Guarantee for Anti-CAMKK2 Antibody covers its use in the following applications.Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Human, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Mouse Brain Tissue Lysate
Lane 4: Mouse Brain Tissue Lysate
IHC(P): Rat Brain Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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