Anti-CAPON/NOS1AP Antibody Picoband™

Product Name

Anti-CAPON/NOS1AP Antibody Picoband™

View all NOS1AP Antibodies

SKU/Catalog Number

A03060-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CAPON/NOS1AP Antibody Picoband™ catalog # A03060-3. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-CAPON/NOS1AP Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03060-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human CAPON/NOS1AP recombinant protein (Position: D24-D438).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03060-3 is reactive to NOS1AP in Human, Mouse, Rat

Applications

A03060-3 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

60 kDa

Calculated molecular weight

56.15kDa

Background of NOS1AP

Nitric oxide synthase 1 adaptor protein (NOS1AP) also known as carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein (CAPON) is a protein that in humans is encoded by the NOS1AP gene. This gene encodes a cytosolic protein that binds to the signaling molecule, neuronal nitric oxide synthase (nNOS). This protein has a C-terminal PDZ-binding domain that mediates interactions with nNOS and an N-terminal phosphotyrosine binding (PTB) domain that binds to the small monomeric G protein, Dexras1. Studies of the related mouse and rat proteins have shown that this protein functions as an adapter protein linking nNOS to specific targets, such as Dexras1 and the synapsins. Alternative splicing results in multiple transcript variants encoding different isoforms

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of CAPON/NOS1AP using anti-CAPON/NOS1AP antibody (A03060-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human Hacat whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAPON/NOS1AP antigen affinity purified polyclonal antibody (Catalog # A03060-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAPON/NOS1AP at approximately 60 kDa. The expected band size for CAPON/NOS1AP is at 56 kDa.

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Figure 2. IHC analysis of CAPON/NOS1AP using anti-CAPON/NOS1AP antibody (A03060-3).
CAPON/NOS1AP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CAPON/NOS1AP Antibody (A03060-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Figure 3. IHC analysis of CAPON/NOS1AP using anti-CAPON/NOS1AP antibody (A03060-3).
CAPON/NOS1AP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CAPON/NOS1AP Antibody (A03060-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Figure 4. Flow Cytometry analysis of HEL cells using anti-CAPON/NOS1AP antibody (A03060-3).
Overlay histogram showing HEL cells stained with A03060-3 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CAPON/NOS1AP Antibody (A03060-3, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For NOS1AP (Source: Uniprot.org, NCBI)

Alternative Names

6330408P19Rik; CAPONMGC138500; carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein; C-terminal PDZ ligand of neuronal nitric oxide synthase protein; KIAA0464C-terminal PDZ domain ligand of neuronal nitric oxide synthase (CAPON); ligand of neuronal nitric oxide synthase with carboxyl-terminal PDZ domain; nitric oxide synthase 1 (neuronal) adaptor protein; Nitric oxide synthase 1 adaptor protein NOS1AP 6330408P19Rik, CAPON, NPHS22 nitric oxide synthase 1 adaptor protein carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein|C-terminal PDZ domain ligand of neuronal nitric oxide synthase (CAPON)|C-terminal PDZ ligand of neuronal nitric oxide synthase protein|ligand of neuronal nitric oxide synthase with carboxyl-terminal PDZ domain|nitric oxide synthase 1 (neuronal) adaptor protein

For more info on NOS1AP, check out the NOS1AP Infographic

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In this infographic, you will see the following information for NOS1AP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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