Anti-Carbonic anhydrase 10/CA10 Antibody Picoband™

Product Name

Anti-Carbonic anhydrase 10/CA10 Antibody Picoband™

View all Carbonic Anhydrase X/CA10 Antibodies

SKU/Catalog Number

A07237-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Carbonic anhydrase 10/CA10 Antibody Picoband™ catalog # A07237-1. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Carbonic anhydrase 10/CA10 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07237-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Carbonic anhydrase 10/CA10 recombinant protein (Position: M1-K328).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A07237-1 is reactive to CA10 in Human

Applications

A07237-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

38 kDa

Calculated molecular weight

37.563kDa

Background of Carbonic Anhydrase X/CA10

Carbonic anhydrase-related protein 10 is an enzyme that in humans is encoded by the CA10 gene. This gene encodes a protein that belongs to the carbonic anhydrase family of zinc metalloenzymes, which catalyze the reversible hydration of carbon dioxide in various biological processes. The protein encoded by this gene is an acatalytic member of the alpha-carbonic anhydrase subgroup, and it is thought to play a role in the central nervous system, especially in brain development. Multiple transcript variants encoding the same protein have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Carbonic anhydrase 10/CA10 using anti-Carbonic anhydrase 10/CA10 antibody (A07237-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Carbonic anhydrase 10/CA10 antigen affinity purified polyclonal antibody (Catalog # A07237-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Carbonic anhydrase 10/CA10 at approximately 38 kDa. The expected band size for Carbonic anhydrase 10/CA10 is at 38 kDa.

Gene/Protein Information For CA10 (Source: Uniprot.org, NCBI)

Alternative Names

CA10; Carbonic Anhydrase X; carbonic anhydrase-related protein 10; Carbonic anhydrase-related protein X; CARP X; CARPX; CA-RPX; CARPXCA-RP X; Cerebral protein 15; cerebral protein-15; HUCEP-15 CA10 CA-RPX, CARPX, HUCEP-15 carbonic anhydrase 10 carbonic anhydrase-related protein 10|CA-RP X|CARP X|carbonic anhydrase X|carbonic anhydrase-related protein X|cerebral protein-15|epididymis secretory sperm binding protein

For more info on CA10, check out the CA10 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CA10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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