Product Info Summary
SKU: | M30929-2 |
---|---|
Size: | 100ul |
Reactive Species: | Streptococcus pyogenes |
Host: | Mouse |
Application: | IHC, WB |
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Product info
Product Name
Anti-CAS9 N-terminal Monoclonal Antibody
SKU/Catalog Number
M30929-2
Size
100ul
Description
Boster Bio Anti-CAS9 N-terminal Monoclonal Antibody catalog # M30929-2. Tested in IHC, WB applications. This antibody reacts with Streptococcus pyogenes.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CAS9 N-terminal Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30929-2)
Host
Mouse
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Monoclonal
Immunogen
N-terminal region, amino acids 1-608 of Cas9 sequence CDJ55032.1 from Streptococcus pyogenes, expressed in and purified from E. coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M30929-2 is reactive to in Streptococcus pyogenes
Applications
M30929-2 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
163571 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1: 2,000 on CAS9 transfected cells and 1:10,000-20,000 on pure full length CAS9 protein. IF/ICC:1:1,000-2,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of M30929-2. [1] Protein size marker with size in kiloDaltons, [2] blot of crude lysate from HEK293 cells transfected with the M30929-2 immunogen, the N-terminal 1-608 amino acids of Cas9, [3] non-transfected control HEK293 cell extract, [4] 40ng full length S. pyogenes Cas9. Blots were probed with M30929-2 at 1:1,000 dilution and as expected, the antibody recognizes the immunogen and full length Cas9 S. pyogenes protein at 160kDa.
Click image to see more details
HEK293 cells were transfected with a construct including the N-terminal 608 amino acids of S. pyogenes CAS9 fused to GFP and stained with M30929-2 in red. Transfected cells express the green fusion protein and bind the antibody in red, producing a yellow signal. Nuclear DNA in transfected and non-transfected cells is revealed with the blue DNA stain DAPI.
Specific Publications For Anti-CAS9 N-terminal Monoclonal Antibody (M30929-2)
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No publications found for M30929-2
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