Anti-Caspase-5 CASP5 Antibody

Product Name

Anti-Caspase-5 CASP5 Antibody

View all CASP5 Antibodies

SKU/Catalog Number

A05259-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Caspase-5 CASP5 Antibody (Catalog # A05259-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

Caspase-5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Caspase-5 CASP5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05259-1)

Host

Rabbit

Contents

Caspase-5 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Caspase-5 antibody was raised against a 14 amino acid synthetic peptide from the center of human Caspase-5. The immunogen is located within amino acids 170 - 220 of Caspase-5.

Cross-reactivity

Depending on cell lines or tissues used, other cleavage products may be observed.

Reactive Species

A05259-1 is reactive to CASP5 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

49.7 kDa

Background of CASP5

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1β and IL-18. Caspase-5 can interact with caspase-1; both are constituents of the NALP1 inflammasome, a complex that can trigger the cleavage of pro-IL-1β. Expression of caspase-5 can be regulated by lipopolysaccharide (LPS) and IFN-gamma.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05259-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Casp-5 antibody can be used for the detection of caspase-5 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information CASP5

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