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Product Info Summary
| SKU: | M02120 |
|---|---|
| Size: | 100 µg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC, WB, FACS |
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Product info
Product Name
Anti-CD73 Antibody (Monoclonal, ABM40E2)
SKU/Catalog Number
M02120
Size
100 µg
Form
Liquid
Description
Boster Bio Anti-CD73 Antibody (Monoclonal, ABM40E2) (Catalog# M02120). Tested in IHC, FACS, WB application(s). This antibody reacts with Human.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-CD73 Antibody (Monoclonal, ABM40E2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02120)
Host
Mouse
Contents
100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide.
Clonality
Monoclonal
Clone Number
ABM40E2
Isotype
IgG2a Kappa
Immunogen
A partial length recombinant CD73 protein (amino acids 145-346)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M02120 is reactive to NT5E in Human
Applications
M02120 is guaranteed for IHC, WB, FACS Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
63.368kDa
Background of 5&
The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. This monoclonal antibody recognizes a protein of 83kDa, which is identified as Factor XIIIa. It has been identified in platelets, megakaryocytes, and fibroblast-like mesenchymal or histiocytic cells in the placenta, uterus, and prostate, monocytes and macrophages and dermal dendritic cells. Anti-factor XIIIa has been found to be useful in differentiating between dermatofibroma (almost all cases are positive), dermatofibrosarcoma protuberans (-/+) and desmoplastic malignant melanoma (-). Anti-factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor, and meningioma.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot: 2-4 µg/ml
IHC: 5 µg/ml, FACS: 0.2-0.5 µg/10^6 cells
Validation Images & Assay Conditions
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Western blot analysis of CD73. Anti-CD73 antibody (Clone: ABM40E2) was tested at 2 µg/ml on human liver lysate.
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Immunohistochemical analysis of CD73 in human Spleen using CD73 antibody (Clone: ABM40E2) at 5 µg/ml.
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Immunohistochemical analysis of CD73 in endomatrium carcinoma using CD73 antibody (Clone: ABM40E2) at 5 µg/ml.
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Immunohistochemical analysis of CD73 in human Liver using CD73 antibody (Clone: ABM40E2) at 5 µg/ml.
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Immunohistochemical analysis of CD73 in renal cell carcinoma using CD73 antibody (Clone: ABM40E2) at 5 µg/ml.
Protein Target Info & Infographic
Gene/Protein Information For NT5E (Source: Uniprot.org, NCBI)
Gene Name
NT5E
Full Name
5'-nucleotidase
Weight
63.368kDa
Superfamily
5'-nucleotidase family
Alternative Names
5' nucleotidase (CD73); 5'-NT; 5-NT; 5'-Nucleotidase; 5'-nucleotidase, ecto (CD73); CD73 antigen; CD73; E5NT; EC 3.1.3.5; ecto-5'-nucleotidase; eN; eNT; NT; NT55'-nucleotidase; NT5E; NTE; Purine 5-Prime-Nucleotidase NT5E CALJA, CD73, E5NT, NT, NT5, NTE, eN, eNT 5-nucleotidase ecto 5-nucleotidase|5-NT|Purine 5-Prime-Nucleotidase|ecto-5-nucleotidase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NT5E, check out the NT5E Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NT5E: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD73 Antibody (Monoclonal, ABM40E2) (M02120)
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No publications found for M02120
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