Product Info Summary
SKU: | A03038-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-Cdc34 Antibody
View all UBE2R1/CDC34 Antibodies
SKU/Catalog Number
A03038-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Cdc34 Antibody catalog # A03038-1. Tested in WB,ICC/IF,IP,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cdc34 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03038-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human HRT2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03038-1 is reactive to CDC34 in Human, Mouse, Rat
Applications
A03038-1 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
26.737kDa
Background of UBE2R1/CDC34
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity By similarity.
Michele A. Battle, PNAS, May 2006; 103: 8419 - 8424.
Masahiko Itoh J. Cell Biol., Dec 1999; 147: 1351.
Salah Amasheh, J. Cell Sci., Dec 2002; 115: 4969.
Evemie Dub¨¦, Biol Reprod, Apr 2007; 10.1095/biolreprod.106.059246.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:5,000
FC: 1:50-1:100
ICC: 1:100-1:500
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of CDC34 using Anti-Cdc34 Antibody (A03038-1).
ICC staining Cdc34 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-Cdc34 Antibody A03038-1
Western blot analysis of Cdc34 on different lysates using anti-Cdc34 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For CDC34 (Source: Uniprot.org, NCBI)
Gene Name
CDC34
Full Name
Ubiquitin-conjugating enzyme E2 R1
Weight
26.737kDa
Superfamily
ubiquitin-conjugating enzyme family
Alternative Names
Cdc34; cell division cycle 34 homolog (S. cerevisiae); cell division cycle 34; E2-CDC34; EC 6.3.2.19; UBC3; UBE2R1; UBE2R1UBCH3; ubiquitin carrier protein; ubiquitin-conjugating enzyme E2 R1; Ubiquitin-conjugating enzyme E2-32 kDa complementing; Ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-protein ligase R1 CDC34 E2-CDC34, UBC3, UBCH3, UBE2R1 cell division cycle 34, ubiqiutin conjugating enzyme ubiquitin-conjugating enzyme E2 R1|(E3-independent) E2 ubiquitin-conjugating enzyme R1|E2 ubiquitin-conjugating enzyme R1|cell division cycle 34 homolog|ubiquitin carrier protein|ubiquitin-conjugating enzyme E2-32 KDA complementing|ubiquitin-conjugating enzyme E2-CDC34|ubiquitin-protein ligase R1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDC34, check out the CDC34 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDC34: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cdc34 Antibody (A03038-1)
Hello CJ!
No publications found for A03038-1
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