Product Info Summary
SKU: | A06361-2 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Centrosomal protein of 135 kDa CEP135 Antibody
SKU/Catalog Number
A06361-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Centrosomal protein of 135 kDa CEP135 Antibody catalog # A06361-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Centrosomal protein of 135 kDa CEP135 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06361-2)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human CEP135
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06361-2 is reactive to CEP135 in Human, Mouse
Applications
A06361-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
133.49kDa
Background of CEP135
Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role.
Fuerst D.O., J. Cell Sci. 102:769-778(1992).
Weber F.E., Eur. J. Biochem. 216:661-669(1993).
Bechtel S., BMC Genomics 8:399-399(2007).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
Validation Images & Assay Conditions
Click image to see more details
Figure 3. Immunohistochemistry validation of CEP135 using Anti-Centrosomal protein of 135 kDa CEP135 Antibody (A06361-2).
Immunohistochemical analysis of paraffin-embedded Human brain using Anti-CEP135 polyclonal antibody. Antibody was diluted at 1:100 (4°CClick image to see more details
Figure 1. Western blotting validation for Anti-Centrosomal protein of 135 kDa CEP135 Antibody A06361-2
Western Blot (WB) analysis of Mouse LungClick image to see more details
Figure 2. Western blotting validation for Anti-Centrosomal protein of 135 kDa CEP135 Antibody A06361-2
Western Blot (WB) analysis of COLO205 cells using Anti-CEP135 polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CEP135 (Source: Uniprot.org, NCBI)
Gene Name
CEP135
Full Name
Centrosomal protein of 135 kDa
Weight
133.49kDa
Superfamily
CEP135/TSGA10 family
Alternative Names
Centrosomal protein of 135 kDa CEP135 CEP4, KIAA0635, MCPH8 centrosomal protein 135 centrosomal protein of 135 kDa|centrosomal protein 135kDa|centrosomal protein 4|centrosome protein cep135
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CEP135, check out the CEP135 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CEP135: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Centrosomal protein of 135 kDa CEP135 Antibody (A06361-2)
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No publications found for A06361-2
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