Anti-Chromogranin A / CHGA (Neuroendocrine Marker) Monoclonal Antibody

Product Name

Anti-Chromogranin A / CHGA (Neuroendocrine Marker) Monoclonal Antibody

View all Chromogranin A Antibodies

SKU/Catalog Number

M01256

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Chromogranin A / CHGA (Neuroendocrine Marker) Monoclonal Antibody (Catalog # M01256). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Monkey, Pig, Mouse, Rat.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Chromogranin A / CHGA (Neuroendocrine Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01256)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM339

Isotype

IgG1, kappa

Immunogen

Human pheochromocytoma

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01256 is reactive to CHGA in Human, Monkey, Mouse, Pig, Rat

Applications

M01256 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

50.688kDa

Background of Chromogranin A

Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Small Cell Lung Carcinoma stained with Anti-Chromogranin A Monoclonal Antibody (SPM339)

Gene/Protein Information For CHGA (Source: Uniprot.org, NCBI)

Alternative Names

betagranin (N-terminal fragment of chromogranin A); Betagranin; Catestatin; CGA; CHGA; Chromofungin; chromogranin A (parathyroid secretory protein 1); Chromogranin A; chromogranin-A; Pancreastatin; Parastatin; Parathyroid Secretory Protein 1; Pituitary Secretory Protein I; SP-I CHGA CGA chromogranin A chromogranin-A|SP-I|betagranin (N-terminal fragment of chromogranin A)|catestatin|chromofungin|parathyroid secretory protein 1|pituitary secretory protein I

For more info on CHGA, check out the CHGA Infographic

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In this infographic, you will see the following information for CHGA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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