Anti-CK16 KRT16 Monoclonal Antibody

Product Name

Anti-CK16 KRT16 Monoclonal Antibody

View all Cytokeratin 16 Antibodies

SKU/Catalog Number

M03393-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-CK16 KRT16 Monoclonal Antibody catalog # M03393-1. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CK16 KRT16 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03393-1)

Host

Mouse

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Monoclonal

Clone Number

6F6

Isotype

IgG

Immunogen

Synthetic Peptide

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

M03393-1 is reactive to KRT16 in Human, Mouse, Rat

Applications

M03393-1 is guaranteed for IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

51.268kDa

Background of Cytokeratin 16

Anti Mesothelin Antibody recognizes Mesothelin that is a glycosyl-phosphatidylinositol–anchored glycoprotein present on the cell surface of various human solid tumors. The mesothelin (MSLN) gene encodes a 71-kDa precursor protein that is processed to a 40-kDa glycosylphosphatidylinositol–anchored protein that composes the mature portion and an NH2 terminal 31-kDa fragment called megakaryocyte-potentiating factor that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues, such as mesothelium, but aberrantly over expressed in mesotheliomas, ovarian, and pancreatic cancers. The biological functions of mesothelin remain elusive. A recent study showed that mesothelin binds to MUC16/CA125, and that this interaction mediates cell adhesion, suggesting that there may be an important role for MUC16/CA125 and mesothelin in the metastatic spread of ovarian cancer.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IHC 1:200

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Immunohistochemistry validation of KRT16 using Anti-CK16 KRT16 Monoclonal Antibody (M03393-1).

Immunohistochemistry (IHC) staining of human gullet cancer tissue

Click image to see more details

Figure 10. Immunohistochemistry validation of KRT16 using Anti-CK16 KRT16 Monoclonal Antibody (M03393-1).

Immunohistochemical analysis of rat kidney tissue. M03393-1 was diluted at 1:200 (4°C

Click image to see more details

Figure 11. Immunohistochemistry validation of KRT16 using Anti-CK16 KRT16 Monoclonal Antibody (M03393-1).

Immunohistochemical analysis of rat lung tissue. M03393-1 was diluted at 1:200 (4°C

Click image to see more details

Figure 2. Immunohistochemistry validation of KRT16 using Anti-CK16 KRT16 Monoclonal Antibody (M03393-1).

Immunohistochemical analysis of human-colon tissue. Anti-CK16 at 1:200 (4°C

Click image to see more details

Figure 3. Immunohistochemistry validation of KRT16 using Anti-CK16 KRT16 Monoclonal Antibody (M03393-1).

Immunohistochemical analysis of human liver cancer tissue. Anti-CK16 at 1:200 (4°C

Click image to see more details

Figure 4. Immunohistochemistry validation of KRT16 using Anti-CK16 KRT16 Monoclonal Antibody (M03393-1).

Immunohistochemical analysis of human liver tissue. Anti-CK16 at 1:200 (4°C

Click image to see more details

Figure 5. Immunohistochemistry validation of KRT16 using Anti-CK16 KRT16 Monoclonal Antibody (M03393-1).

Immunohistochemical analysis of human lung cancer tissue. Anti-CK16 at 1:200 (4°C

Click image to see more details

Figure 6. Immunohistochemistry validation of KRT16 using Anti-CK16 KRT16 Monoclonal Antibody (M03393-1).

Immunohistochemical analysis of human lung tissue. Anti-CK16 at 1:200 (4°C

Click image to see more details

Figure 7. Immunohistochemistry validation of KRT16 using Anti-CK16 KRT16 Monoclonal Antibody (M03393-1).

Immunohistochemical analysis of human stomach cancer tissue. Anti-CK16 at 1:200 (4°C

Click image to see more details

Figure 8. Immunohistochemistry validation of KRT16 using Anti-CK16 KRT16 Monoclonal Antibody (M03393-1).

Immunohistochemical analysis of human stomach tissue. M03393-1 was diluted at 1:200 (4°C

Click image to see more details

Figure 9. Immunohistochemistry validation of KRT16 using Anti-CK16 KRT16 Monoclonal Antibody (M03393-1).

Immunohistochemical analysis of human tonsil tissue. M03393-1 was diluted at 1:200 (4°C

Gene/Protein Information For KRT16 (Source: Uniprot.org, NCBI)

Alternative Names

CK16; CK-16; cytokeratin 16; cytokeratin-16; FNEPPK; focal non-epidermolytic palmoplantar keratoderma; K16; K1CP; keratin 16; keratin, type I cytoskeletal 16; keratin-16; KRT16A; NEPPK KRT16 CK16, FNEPPK, K16, K1CPA, NEPPK, PC1, KRT16 keratin 16 keratin, type I cytoskeletal 16|cytokeratin 16|focal non-epidermolytic palmoplantar keratoderma|keratin 16, type I

For more info on KRT16, check out the KRT16 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for KRT16: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the KRT16 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis