Click image to see more details
Product Info Summary
| SKU: | M03134-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Cow, Human, Mouse, Pig, Rat |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IP, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Clathrin Heavy Chain Purified CLTC Monoclonal Antibody
SKU/Catalog Number
M03134-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Clathrin Heavy Chain Purified CLTC Monoclonal Antibody (Catalog# M03134-1). Tested in Flow Cytometry, IP, WB, ICC, ELISA application(s). This antibody reacts with Rat, Cow, Mouse, Human, Pig.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Clathrin Heavy Chain Purified CLTC Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03134-1)
Host
Mouse
Contents
Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
BF-06
Isotype
Mouse IgM
Immunogen
The antibody BF-06 recognizes clathrin heavy chain, an ubiquitously expressed 180 kDa intracellular protein involved in receptor-mediated endocytosis.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M03134-1 is reactive to CTLC in Cow, Human, Mouse, Pig, Rat
Observed Molecular Weight
42 kDa
Calculated molecular weight
191.6 kDa
Background of CTLC
Clathrin is a submembrane protein that polymerizes into coat-like lattices, which results in membrane invagination. The basic oligomers are composed of three clathrin heavy chain (180 kDa) and three light chain (30 kDa) subunits and the process of polymerization is dynamically regulated by the light chains. Interaction of clathrin with the plasma membrane is mediated by adaptor proteins (AP1-4) specific for different cellular compartments. Another proteins, such as endophilin, epsin and amphiphysin are involved in membrane invagination and clathrin rearrangements. Finally, dynamin functions at the fission stage of clathrin-mediated endocytosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03134-1 is guaranteed for ELISA, Flow Cytometry, IP, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 2-5 μg/ml; positive control: human blood leukocytes. Intracellular staining.
Validation Images & Assay Conditions
Click image to see more details
Separation of MCF-7 cells stained using anti-Clathrin (BF-06) purified antibody (concentration in sample 2 µg/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).
Click image to see more details
Immunocytochemistry staining of clathrin in human primary fibroblasts using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
Click image to see more details
Immunocytochemistry staining of clathrin in human HeLa cell line using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
Specific Publications For Anti-Clathrin Heavy Chain Purified CLTC Monoclonal Antibody (M03134-1)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Clathrin Heavy Chain Purified CLTC Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Clathrin Heavy Chain Purified CLTC Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
