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Product Info Summary
| SKU: | M05840 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-CLIC5 Purified Monoclonal Antibody
SKU/Catalog Number
M05840
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CLIC5 Purified Monoclonal Antibody (Catalog# M05840). Tested in Flow Cytometry, WB application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-CLIC5 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05840)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
CLIC5-02
Isotype
Mouse IgG2b kappa
Immunogen
KLH-conjugated peptide corresponding to the amino acids 160-173 of human CLIC5a (Cys-Ile-Asp-Ala-Asn-Thr-Ser-Asp-Lys-Gly-Ser-Arg-Arg coupled with KLH). Mouse monoclonal antibody CLIC5-02 recognizes CLIC5a, a 32 kDa intracellular protein which associates with proteins of actin complexes.
Cross-reactivity
Cross-reactivity with CLIC5b was not determined.
Reactive Species
M05840 is reactive to CLIC5 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
46.5 kDa
Background of CLIC5
CLIC5a belongs to the family of chloride intracellular channel (CLIC) proteins, all sharing a highly conserved C terminus and variable N terminus. Human CLIC5 is transcribed in two isoforms, 32 kDa CLIC5a (251 amino acids) and 49 kDa CLIC5b (410 amino acids). These proteins exist in a soluble form and their function as ion channels in vitro has not been fully confirmed in vivo. CLIC5a is a component of complexes of actin, ezrin, and several other actin-associated proteins and is important for functionality of actin-based structures.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05840 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml. Intracellular staining.
Validation Images & Assay Conditions
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Western blotting analysis of CLIC5a in HEK293-CLIC5a transfectants (A) and HEK293 cells (B) using mouse monoclonal antibody (clone CLIC5-02).
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Separation of NALM-6 cells stained using anti-CLIC5 (CLIC5-02) purified antibody (concentration in sample 5 µg/ml, GAM APC, red-filled) from NALM-6 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).
Specific Publications For Anti-CLIC5 Purified Monoclonal Antibody (M05840)
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