Anti-Cortactin CTTN Rabbit Monoclonal Antibody

Product Name

Anti-Cortactin CTTN Rabbit Monoclonal Antibody

View all Cortactin Antibodies

SKU/Catalog Number

M01253

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Cortactin CTTN Rabbit Monoclonal Antibody catalog # M01253. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cortactin CTTN Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01253)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

FAI-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Cortactin

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01253 is reactive to CTTN in Human, Mouse, Rat

Applications

M01253 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

85 kDa

Calculated molecular weight

61.586kDa

Background of Cortactin

Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:5000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:20
FC 1:20

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Cortactin using anti-Cortactin antibody (M01253).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cortactin antigen affinity purified monoclonal antibody (Catalog # M01253) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cortactin at approximately 85 kDa. The expected band size for Cortactin is at 62 kDa.

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Immunohistochemical analysis of paraffin-embedded human breast cancer, using Cortactin Antibody.

Gene/Protein Information For CTTN (Source: Uniprot.org, NCBI)

Alternative Names

1110020L01Rik; Amplaxin; Cortactin; CTTN; ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 srcsubstrate); EMS1; EMS1amplaxin; FLJ34459; Oncogene EMS1; src substrate cortactin CTTN EMS1 cortactin src substrate cortactin|1110020L01Rik|amplaxin|ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate)|epididymis secretory sperm binding protein|oncogene EMS1

For more info on CTTN, check out the CTTN Infographic

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In this infographic, you will see the following information for CTTN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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