Anti-CRYAB/Alpha B Crystallin Antibody

Product Name

Anti-CRYAB/Alpha B Crystallin Antibody

View all AlphaB Crystallin/CRYAB Antibodies

SKU/Catalog Number

A03496

Size

50 µg

Form

Liquid

Description

Boster Bio Anti-CRYAB/Alpha B Crystallin Antibody catalog # A03496. Tested in WB, IP applications. This antibody reacts with Mouse, Rat, Bovine, Porcine, Sheep.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CRYAB/Alpha B Crystallin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03496)

Host

Goat

Contents

In sodium phosphate buffer, pH 7.0, containing 0.1% sodium azide, 3% BSA, and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic peptide corresponding to to aa 54-64 of of human αB-crystallin phosphorylated at Ser59.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A03496 is reactive to CRYAB in Bovine, Mouse, Rat, Sheep, Porcine

Applications

A03496 is guaranteed for IP, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

20.159kDa

Background of AlphaB Crystallin/CRYAB

The laminar organization of multiple neuronal types in the cerebral cortex is required for normal cognitive function. In mice, the disabled-1 gene plays a central role in brain development, directing the migration of cortical neurons past previously formed neurons to reach their proper layer. This gene is similar to disabled-1, and the protein encoded by this gene is thought to be a signal transducer that interacts with protein kinase pathways to regulate neuronal positioning in the developing brain. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined.

Kelian Chen, et,al. (2003) J. Cell Sci ; 117: 4527 - 4536.
Vera Strasser, et,al. (2004) Mol. Cell. Biol ; 24: 1378 - 1386.
Izhar Ben-Shlomo, et,al. (2003) Sci. STKE ; 2003: 9.
H. M. Kim, et,al. (2002) PNAS ; 99: 4020.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, ECL, 1:1, 000
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

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Western blot analysis of PSer 59 Alpha B-Crystallin expression in MW marker (lane 1), native bovine α-Crystallin protein (lane 2), native bovine β-Crystallin protein (lane 3), mouse heart extract (lane 4), rat heart extract (lane 5) and ESK-4 whole cell lysates (lane 6). PSer 59 Alpha B-Crystallin was detected using rabbit anti-PSer 59 Alpha B-Crystallin polyclonal antibody (Catalog # A03496) at 1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

Gene/Protein Information For CRYAB (Source: Uniprot.org, NCBI)

Alternative Names

alpha B crystallin; alpha(B)-crystallin; AlphaB Crystallin; alpha-crystallin B chain; CRYA2; CRYA2alpha crystallin B chain; CRYAB; crystallin, alpha B; CTPP2; Heat shock protein beta-5; heat-shock 20 kD like-protein; HSPB5; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component CRYAB CMD1II, CRYA2, CTPP2, CTRCT16, HEL-S-101, HSPB5, MFM2 crystallin alpha B alpha-crystallin B chain|epididymis secretory protein Li 101|heat shock protein beta-5|heat-shock 20 kD like-protein|renal carcinoma antigen NY-REN-27|rosenthal fiber component

For more info on CRYAB, check out the CRYAB Infographic

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In this infographic, you will see the following information for CRYAB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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