Anti-Cyt 19/As3mt Antibody Picoband™

Product Name

Anti-Cyt 19/As3mt Antibody Picoband™

View all As3mt Antibodies

SKU/Catalog Number

A05107-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cyt 19/As3mt Antibody Picoband™ catalog # A05107-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cyt 19/As3mt Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05107-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse Cyt 19/As3mt recombinant protein (Position: M1-D346).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05107-2 is reactive to As3mt in Mouse

Applications

A05107-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

44 kDa

Calculated molecular weight

41.794kDa

Background of As3mt

AS3MT catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (AdoMet) to trivalent arsenical and may play a role in arsenic metabolism. This gene is mapped to chromosome 10q24.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Mouse
Flow Cytometry, 1-3μg/1x10⁶ cells, Mouse
Direct ELISA, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Cyt 19/As3mt using anti-Cyt 19/As3mt antibody (A05107-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse liver tissue lysates,
Lane 2: mouse heart tissue lysates,
Lane 3: mouse Neuro-2a whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyt 19/As3mt antigen affinity purified polyclonal antibody (Catalog # A05107-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyt 19/As3mt at approximately 44KD. The expected band size for Cyt 19/As3mt is at 42KD.

Click image to see more details

Figure 2. Flow Cytometry analysis of mouse spleen tissues using anti-Cyt 19/As3mt antibody (A05107-2).
Overlay histogram showing A431 cells stained with A05107-2 (Blue line).The tissues were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cyt 19/As3mt Antibody (A05107-2,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For As3mt (Source: Uniprot.org, NCBI)

Alternative Names

Arsenic (+3 Oxidation State) Methyltransferase; Arsenic Methyltransferase; arsenite methyltransferase; AS3MT; CYT19; CYT19methyltransferase cyt19; EC 2.1.1.137; Methylarsonite Methyltransferase; Methyltransferase Cyt19; S-Adenosyl-L-Methionine:Arsenic(III) Methyltransfe; S-adenosyl-L-methionine:arsenic(III) methyltransferase; S-Adenosylmethionine:Arsenic (III) Methyltransfera; S-adenosylmethionine:arsenic (III) methyltransferase As3mt|2310045H08Rik, Cyt19|arsenite methyltransferase|arsenite methyltransferase|S-adenosyl-L-methionine:arsenic(III) methyltransferase|S-adenosylmethionine:arsenic (III) methyltransferase|arsenic (+3 oxidation state) methyltransferase|methylarsonite methyltransferase|methyltransferase Cyt19

For more info on As3mt, check out the As3mt Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for As3mt: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the As3mt Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis