Anti-Cytokeratin, pan (Epithelial Marker) Antibody

Product Name

Anti-Cytokeratin, pan (Epithelial Marker) Antibody

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SKU/Catalog Number

M30944

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Cytokeratin, pan (Epithelial Marker) Antibody (Catalog # M30944). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Monkey, Cow, Dog, Rabbit, Mouse, Rat, Cat, Chicken.

Storage & Handling

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Cytokeratin, pan (Epithelial Marker) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30944)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: PAN-CK (Cocktail)

Isotype

IgG s, kappa

Immunogen

Human epidermal keratins

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M30944 is reactive to in Chicken, Cow, Dog, Human, Monkey, Mouse, Rabbit, Rat, Cat

Applications

M30944 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

51386 MW

Background of

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, with 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 55kDa (CK7); 52kDa (CK8); 56.5kDa (CK10); 53kDa (CK13); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 46kDa (CK17); 45kDa (CK18) and 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRT-PAN is a broad-spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It is useful in characterizing the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and shows high sensitivity in the recognition of epithelial cells and carcinomas.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (0.5-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (0.5-2ug/ml for 2 hours at RT)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human basal cell carcinoma stained with Anti-Cytokeratin, Pan Mouse Monoclonal Antibody (PAN-CK).

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Formalin-fixed, paraffin-embedded human squamous cell carcinoma stained with Anti-Cytokeratin, Pan Mouse Monoclonal Antibody (PAN-CK).

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Confocal immunofluorescent analysis of HeLa cells using Anti-Cytokeratin, Pan Mouse Monoclonal Antibody (PAN-CK) followed by goat anti-mouse IgG-CF488 (cyan). Nuclei are counterstained with NucSpot (red).

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Flow cytometric analysis of human HeLa cells. Anti-Cytokeratin, Pan Mouse Monoclonal Antibody (PAN-CK) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

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