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Product Info Summary
| SKU: | M00670 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-DDX5 Monoclonal Antibody
SKU/Catalog Number
M00670
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-DDX5 Monoclonal Antibody catalog # M00670. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DDX5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00670)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADBC-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human DDX5 RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00670 is reactive to DDX5 in Human, Mouse, Rat
Applications
M00670 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
54 kDa
Calculated molecular weight
69.148kDa
Background of DDX5
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
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Western blot analysis of DDX5 expression in HeLa cell lysate.
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All lanes use the Antibody at 1:8K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:8K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:8K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For DDX5 (Source: Uniprot.org, NCBI)
Gene Name
DDX5
Full Name
Probable ATP-dependent RNA helicase DDX5
Weight
69.148kDa
Superfamily
DEAD box helicase family
Alternative Names
B-cell CLL/lymphoma 7 protein family member C; B-cell CLL/lymphoma 7C; DDX5; DEAD/H; G17P1; HELR; HLR1; HUMP68 DDX5 G17P1, HLR1, HUMP68, p68 DEAD-box helicase 5 probable ATP-dependent RNA helicase DDX5|ATP-dependent RNA helicase DDX5|DEAD (Asp-Glu-Ala-Asp) box helicase 5|DEAD (Asp-Glu-Ala-Asp) box polypeptide 5|DEAD box protein 5|DEAD box-5|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD)|RNA helicase p68
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DDX5, check out the DDX5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DDX5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DDX5 Monoclonal Antibody (M00670)
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No publications found for M00670
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Customer Reviews
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